ArticleMolecular microbiology2013
Phosphorylation-dependent localization of the response regulator FrzZ signals cell reversals in Myxococcus xanthus.
Article in Molecular microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Milestones in the development ofJournal of bacteriology · 2025Review
- A cyanobacterial chemotaxis-like system controls phototactic orientation via phosphorylation of two antagonistic response regulators.microLife · 2024Article
- A miniTurbo-based proximity labeling protocol to identify conditional protein interactomes in vivo in Myxococcus xanthus.STAR protocols · 2023Article
- Molecular basis and design principles of switchable front-rear polarity and directional migration in Myxococcus xanthus.Nature communications · 2023Article
- Regulation of Sporangium Formation, Spore Dormancy, and Sporangium Dehiscence by a Hybrid Sensor Histidine Kinase in Actinoplanes missouriensis: Relationship with the Global Transcriptional Regulator TcrA.Journal of bacteriology · 2020Article
- Protein-protein interaction network controlling establishment and maintenance of switchable cell polarity.PLoS genetics · 2020Article
- Spatial control of the GTPase MglA by localized RomR-RomX GEF and MglB GAP activities enables Myxococcus xanthus motility.Nature microbiology · 2019Article
- Cyclic-di-GMP binds to histidine kinase RavS to control RavS-RavR phosphotransfer and regulates the bacterial lifestyle transition between virulence and swimming.PLoS pathogens · 2019Article
- A gated relaxation oscillator mediated by FrzX controls morphogenetic movements in Myxococcus xanthus.Nature microbiology · 2018Article
- Evolution and Design Governing Signal Precision and Amplification in a Bacterial Chemosensory Pathway.PLoS genetics · 2015Article
- The Cutting Edge of Affinity Electrophoresis Technology.Proteomes · 2015Review
- The polarity of myxobacterial gliding is regulated by direct interactions between the gliding motors and the Ras homolog MglA.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Dual biochemical oscillators may control cellular reversals in Myxococcus xanthus.Biophysical journal · 2014Article
- Cell division resets polarity and motility for the bacterium Myxococcus xanthus.Journal of bacteriology · 2014Article
- Establishing polar identity in gram-negative rods.Current opinion in microbiology · 2013Review
- Microcompartments and protein machines in prokaryotes.Journal of molecular microbiology and biotechnology · 2013Review
- Structural characterization of Myxococcus xanthus MglC, a component of the polarity control system, and its interactions with its paralog MglB.The Journal of biological chemistryArticle
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2 authors.
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Abstract
The life cycle of Myxococcus xanthus includes co-ordinated group movement and fruiting body formation, and requires directed motility and controlled cell reversals. Reversals are achieved by inverting cell polarity and re-organizing many motility proteins. The Frz chemosensory pathway regulates the frequency of cell reversals. While it has been established that phosphotransfer from the kinase FrzE to the response regulator FrzZ is required, it is unknown how phosphorylated FrzZ, the putative output of the pathway, targets the cell polarity axis. In this study, we used Phos-tag SDS-PAGE to determine the cellular level of phospho-FrzZ under different growth conditions and in Frz signalling mutants. We detected consistent FrzZ phosphorylation, albeit with a short half-life, in cells grown on plates, but not from liquid culture. The available pool of phospho-FrzZ correlated with reversal frequencies, with higher levels found in hyper-reversing mutants. Phosphorylation was not detected in hypo-reversing mutants. Fluorescence microscopy revealed that FrzZ is recruited to the leading cell pole upon phosphorylation and switches to the opposite pole during reversals. These results are consistent with the hypothesis that the Frz pathway modulates reversal frequency through a localized response regulator that targets cell polarity regulators at the leading cell pole.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.