ArticleAlcoholism, clinical and experimental research2013
Intraperitoneal injection of ethanol results in drastic changes in bone metabolism not observed when ethanol is administered by oral gavage.
Article in Alcoholism, clinical and experimental research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 27 citations in OpenAlex.
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- Dose-response effects of alcohol on biochemical markers of bone turnover in non-human primates: Effects of species, sex and age of onset of drinking.Bone reports · 2022Article
- A Traditional Chinese Medicine Plant Extract Prevents Alcohol-Induced Osteopenia.Frontiers in pharmacology · 2021Article
- β-Nicotinamide Mononucleotide (NMN) Administrated by Intraperitoneal Injection Mediates Protection Against UVB-Induced Skin Damage in Mice.Journal of inflammation research · 2021Article
- Making Sense of the Highly Variable Effects of Alcohol on Bone.Clinical reviews in bone and mineral metabolism · 2021Article
- Chlorpyrifos and ΔCurrent research in toxicology · 2021Review
- Bone Effects of Binge Alcohol Drinking Using Prepubescent Pigs as a Model.Alcoholism, clinical and experimental research · 2018Article
- Effects of Intermittent Alcohol Exposure on Emotion and Cognition: A Potential Role for the Endogenous Cannabinoid System and Neuroinflammation.Frontiers in behavioral neuroscience · 2017Article
- Alcohol-induced suppression of KDM6B dysregulates the mineralization potential in dental pulp stem cells.Stem cell research · 2016Article
- Alcohol: A Simple Nutrient with Complex Actions on Bone in the Adult Skeleton.Alcoholism, clinical and experimental research · 2016Review
- Exogenous activation of Wnt/β-catenin signaling attenuates binge alcohol-induced deficient bone fracture healing.Alcohol and alcoholism (Oxford, Oxfordshire)Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
backgroundChronic alcohol abuse is associated with increased risk of osteoporosis while light-to-moderate alcohol intake correlates with reduced osteoporosis risk. Addition of alcohol to a liquid diet is often used to model chronic alcohol abuse. Methods to model intermittent drinking (including binge drinking and light-to-moderate consumption) include (i) intragastric administration of alcohol by oral gavage or (ii) intraperitoneal (ip) administration of alcohol by injection. However, it is unclear whether the latter 2 methods produce comparable results. The purpose of this investigation was to determine the skeletal response to alcohol delivered daily by oral gavage or ip injection.
methodsEthanol (EtOH) or vehicle was administered to 4-month-old female Sprague-Dawley rats once daily at 1.2 g/kg body weight for 7 days. Following necropsy, bone formation and bone architecture were evaluated in tibial diaphysis (cortical bone) and proximal tibial metaphysis (cancellous bone) by histomorphometry. mRNA was measured for bone matrix proteins in distal femur metaphysis.
resultsAdministration of alcohol by gavage had no significant effect on body weight gain or bone measurements. In contrast, administration of the same dose of alcohol by ip injection resulted in reduced body weight, total suppression of periosteal bone formation in tibial diaphysis, decreased cancellous bone formation in proximal tibial metaphysis, and decreased mRNA levels for bone matrix proteins in distal femur.
conclusionsOur findings raise concerns regarding the use of ip injection of EtOH in rodents as a method for modeling the skeletal effects of intermittent exposure to alcohol in humans. This concern is based on a failure of the ip route to replicate the oral route of alcohol administration.
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