ArticleACS nano2013
Designing a polycationic probe for simultaneous enrichment and detection of microRNAs in a nanopore.
Article in ACS nano, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
31 citing papers in PubMed.
- Background-Free Nanopore Decoding of microRNA Expression Patterns Using Circular Diagnostic DNA.Analytical chemistry · 2026Article
- The Role of microRNAs in Lung Cancer: Mechanisms, Diagnostics and Therapeutic Potential.International journal of molecular sciences · 2025Review
- Nanopore Single-molecule Analysis of Biomarkers: Providing Possible Clues to Disease Diagnosis.Trends in analytical chemistry : TRAC · 2023Article
- A nanopore interface for higher bandwidth DNA computing.Nature communications · 2022Article
- The Nanopore-Tweezing-Based, Targeted Detection of Nucleobases on Short Functionalized Peptide Nucleic Acid Sequences.Polymers · 2021Article
- Biological Nanopores: Engineering on Demand.Life (Basel, Switzerland) · 2021Review
- Influence of Electrolyte Concentration on Single-Molecule Sensing of Perfluorocarboxylic Acids.Frontiers in chemistry · 2021Article
- Sequence-Specific Detection of DNA Strands Using a Solid-State Nanopore Assisted by Microbeads.Micromachines · 2020Article
- Analytical Model for Particle Capture in Nanopores Elucidates Competition among Electrophoresis, Electroosmosis, and Dielectrophoresis.ACS nano · 2020Article
- Resistive amplitude fingerprints during translocation of linear molecules through charged solid-state nanopores.The Journal of chemical physics · 2020Article
- Sequence-specific detection of single-stranded DNA with a gold nanoparticle-protein nanopore approach.Scientific reports · 2020Article
- PNA-Based MicroRNA Detection Methodologies.Molecules (Basel, Switzerland) · 2020Review
- Insights into protein sequencing with an α-Hemolysin nanopore by atomistic simulations.Scientific reports · 2019Article
- Technological Challenges and Future Issues for the Detection of Circulating MicroRNAs in Patients With Cancer.Frontiers in chemistry · 2019Review
- The aerolysin nanopore: from peptidomic to genomic applications.Nanoscale · 2018Review
- Label-Free Detection of DNA Mutations by Nanopore Analysis.ACS applied materials & interfaces · 2018Article
- Membrane protein-based biosensors.Journal of the Royal Society, Interface · 2018Review
- Remote Activation of a Nanopore for High-Performance Genetic Detection Using a pH Taxis-Mimicking Mechanism.Analytical chemistry · 2017Article
- Interference-Free Detection of Genetic Biomarkers Using Synthetic Dipole-Facilitated Nanopore Dielectrophoresis.ACS nano · 2017Article
- Polycationic Probe-Guided Nanopore Single-Molecule Counter for Selective miRNA Detection.Methods in molecular biology (Clifton, N.J.) · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
Abstract
The nanopore sensor can detect cancer-derived nucleic acid biomarkers such as microRNAs (miRNAs), providing a noninvasive tool potentially useful in medical diagnostics. However, the nanopore-based detection of these biomarkers remains confounded by the presence of numerous other nucleic acid species found in biofluid extracts. Their nonspecific interactions with the nanopore inevitably contaminate the target signals, reducing the detection accuracy. Here we report a novel method that utilizes a polycationic peptide-PNA probe as the carrier for selective miRNA detection in the nucleic acid mixture. The cationic probe hybridized with microRNA forms a dipole complex, which can be captured by the pore using a voltage polarity that is opposite the polarity used to capture negatively charged nucleic acids. As a result, nontarget species are driven away from the pore opening, and the target miRNA can be detected accurately without interference. In addition, we demonstrate that the PNA probe enables accurate discrimination of miRNAs with single-nucleotide difference. This highly sensitive and selective nanodielectrophoresis approach can be applied to the detection of clinically relevant nucleic acid fragments in complex samples.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.