ArticleCurrent microbiology2013
Analysis of yeast-like symbiote diversity in the brown planthopper (BPH), Nilaparvata lugens Stål, using a novel nested PCR-DGGE protocol.
Article in Current microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 34 citations in OpenAlex.
- Transcriptome Analysis of the Fat Body of the Maize Pest Delphacodes kuscheli (Hemiptera: Delphacidae) Reveals Essential Roles of Fungal Endosymbionts.Microbial ecology · 2025Article
- Environmental yeasts differentially impact the development and oviposition behavior of the Asian tiger mosquito Aedes albopictus.Microbiome · 2025Article
- Bacterial communities inHeliyon · 2025Article
- Friendly fungi: Tropical insect families form partnerships with intracellular fungi related to pathogens.iScience · 2024Article
- Virulence Adaptation by Rice Planthoppers and Leafhoppers to Resistance Genes and Loci: A Review.Insects · 2024Review
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- Diversity and Functions of Yeast Communities Associated with Insects.Microorganisms · 2021Review
- Antimicrobials Affect the Fat Body Microbiome and Increase the Brown Planthopper Mortality.Frontiers in physiology · 2021Article
- The gut bacterial flora associated with brown planthopper is affected by host rice varieties.Archives of microbiology · 2021Article
- Endomycobiome associated with females of the planthopperHeliyon · 2020Article
- Fungicides Reduce the Abundance of Yeast-like Symbionts and Survival of White-Backed PlanthopperInsects · 2020Article
- Article
- Characterization of a Bacterial SymbiontFrontiers in microbiology · 2019Article
- Effects of Fungicide Propiconazole on the Yeast-Like Symbiotes in Brown Planthopper (BPH,Frontiers in physiology · 2019Article
- New insights into the fungal community from the raw genomic sequence data of fig wasp Ceratosolen solmsi.BMC microbiology · 2015Article
- Small, smaller, smallest: the origins and evolution of ancient dual symbioses in a Phloem-feeding insect.Genome biology and evolution · 2013Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Yeast-like symbiotes (YLS) are endosymbionts that are intimately associated with the growth, development, reproduction of their host, the brown planthopper, Nilaparvata lugens (Hemiptera: Delphacidae). However, it is unclear how many species of YLS are found within N. lugens, and how they are related to each other. Traditional methods or simple amplification based on 18S rDNA sequence does not reliably identify new species quickly and efficiently. Therefore, a novel nested PCR-denaturing gradient gel electrophoresis (DGGE) strategy was developed in this article to analyze the YLS of brown planthopper using a nested PCR protocol that involved the 18S rDNA gene and the 5.8S-ITS gene using fungal universal primers. The nested PCR protocol was developed as follows: firstly, the 18S rDNA gene, and 5.8S-ITS gene were amplified using fungal universal primers. Subsequently, these products were used as a template in a second PCR with primers ITS1GC-ITS2, ITS1FGC-ITS2, and NFGC-NR, which was suitable for DGGE. Using this highly specific molecular approach, we found several previously detected fungi: Noda, Pichia guilliermondii, Candida sp., and some previously undetected fungi, such as Saccharomycetales sp., Debaryomyces hansenii, and some uncultured fungi. In conclusion, the nested PCR system developed in this study, coupled with DGGE fingerprinting, offers a new tool for uncovering fungal endosymbiont diversity within planthoppers.
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