ArticleMolecular systems biology2013
Temporal system-level organization of the switch from glycolytic to gluconeogenic operation in yeast.
Article in Molecular systems biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 74 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
74 citing papers in PubMed, 134 citations in OpenAlex.
- Coarse-grained resource allocation modeling for decoding and rewiring microbial metabolism.Trends in microbiology · 2026Review
- Article
- Systematic transcriptomics analysis of calorie restriction and rapamycin unveils their synergistic interaction in prolonging cellular lifespan.Communications biology · 2025Article
- Combined Isotopic Tracer and Modelling Approach Reveals Differences in Nitrogen Metabolism in S. cerevisiae, S. uvarum and S. kudriavzevii Species.Microbial biotechnology · 2025Article
- A four eigen-phase model of multi-omics unveils new insights into yeast metabolic cycle.NAR genomics and bioinformatics · 2025Article
- Rap1 overexpression boosts triterpenoid saponin production in yeast by enhancing precursor supply and heterologous gene expression.Microbial cell factories · 2025Article
- Glucosinolates from Seed-Press Cake ofAntioxidants (Basel, Switzerland) · 2025Article
- A comparative analysis of NADPH supply strategies inMetabolic engineering communications · 2024Article
- A high-resolution two-step evolution experiment in yeast reveals a shift from pleiotropic to modular adaptation.PLoS biology · 2024Article
- Construction of an alternative NADPH regeneration pathway improves ethanol production inSynthetic and systems biotechnology · 2024Article
- Emergence of Orchestrated and Dynamic Metabolism ofACS synthetic biology · 2024Article
- Reversing the directionality of reactions between non-oxidative pentose phosphate pathway and glycolytic pathway boosts mycosporine-like amino acid production in Saccharomyces cerevisiae.Microbial cell factories · 2024Article
- A fast method to distinguish between fermentative and respiratory metabolisms in single yeast cells.iScience · 2024Article
- Controlling circuitry underlies the growth optimization of Saccharomyces cerevisiae.Metabolic engineering · 2023Article
- Sir2 and Glycerol Underlie the Pro-Longevity Effect of Quercetin during Yeast Chronological Aging.International journal of molecular sciences · 2023Article
- Yeast increases glycolytic flux to support higher growth rates accompanied by decreased metabolite regulation and lower protein phosphorylation.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Article
- High-throughput metabolomics for the design and validation of a diauxic shift model.NPJ systems biology and applications · 2023Article
- Endo metabolomic profiling ofFrontiers in microbiology · 2023Article
- Spatial-temporal regulation of fatty alcohol biosynthesis in yeast.Biotechnology for biofuels and bioproducts · 2022Article
14 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The diauxic shift in Saccharomyces cerevisiae is an ideal model to study how eukaryotic cells readjust their metabolism from glycolytic to gluconeogenic operation. In this work, we generated time-resolved physiological data, quantitative metabolome (69 intracellular metabolites) and proteome (72 enzymes) profiles. We found that the diauxic shift is accomplished by three key events that are temporally organized: (i) a reduction in the glycolytic flux and the production of storage compounds before glucose depletion, mediated by downregulation of phosphofructokinase and pyruvate kinase reactions; (ii) upon glucose exhaustion, the reversion of carbon flow through glycolysis and onset of the glyoxylate cycle operation triggered by an increased expression of the enzymes that catalyze the malate synthase and cytosolic citrate synthase reactions; and (iii) in the later stages of the adaptation, the shutting down of the pentose phosphate pathway with a change in NADPH regeneration. Moreover, we identified the transcription factors associated with the observed changes in protein abundances. Taken together, our results represent an important contribution toward a systems-level understanding of how this adaptation is realized.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.