ArticleScientific reports2013
The interactomes of POU5F1 and SOX2 enhancers in human embryonic stem cells.
Article in Scientific reports, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 34 citations in OpenAlex.
- Induced pluripotent stem cell-derived smooth muscle cells to study cardiovascular calcification.Frontiers in cardiovascular medicine · 2022Article
- Roles Played by YY1 in Embryonic, Adult and Cancer Stem Cells.Stem cell reviews and reports · 2021Review
- Long-distance chromatin interaction of IGF1 during embryonic and postnatal development in the liver of Sus scrofa.Functional & integrative genomics · 2021Article
- Exploring trophoblast-specific Tead4 enhancers through chromatin conformation capture assays followed by functional screening.Nucleic acids research · 2020Article
- KLF4 protein stability regulated by interaction with pluripotency transcription factors overrides transcriptional control.Genes & development · 2019Article
- The Role of RNA Polymerase II Contiguity and Long-Range Interactions in the Regulation of Gene Expression in Human Pluripotent Stem Cells.Stem cells international · 2019Article
- Chromatin organization in pluripotent cells: emerging approaches to study and disrupt function.Briefings in functional genomics · 2016Review
- Yin Yang 1 is associated with cancer stem cell transcription factors (SOX2, OCT4, BMI1) and clinical implication.Journal of experimental & clinical cancer research : CR · 2016Review
- 4C-seq revealed long-range interactions of a functional enhancer at the 8q24 prostate cancer risk locus.Scientific reports · 2016Article
- Analysis of a transgenic Oct4 enhancer reveals high fidelity long-range chromosomal interactions.Scientific reports · 2015Article
- Article
- Single-cell gene expression profiles define self-renewing, pluripotent, and lineage primed states of human pluripotent stem cells.Stem cell reports · 2014Article
- The dynamic architectural and epigenetic nuclear landscape: developing the genomic almanac of biology and disease.Journal of cellular physiology · 2014Review
- Computational characterization of modes of transcriptional regulation of nuclear receptor genes.PloS one · 2014Article
- Biological implications and regulatory mechanisms of long-range chromosomal interactions.The Journal of biological chemistry · 2013Review
- Genome-wide chromatin interactions of the Nanog locus in pluripotency, differentiation, and reprogramming.Cell stem cell · 2013Article
- Integrated module and gene-specific regulatory inference implicates upstream signaling networks.PLoS computational biology · 2013Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The genes POU5F1 and SOX2 are critical for pluripotency and reprogramming, yet the chromosomal organization around these genes remains poorly understood. We assayed long-range chromosomal interactions on putative enhancers of POU5F1 and SOX2 genes in human embryonic stem cells (hESCs) using 4C-Seq technique. We discovered that their frequent interacting regions mainly overlap with early DNA replication domains. The interactomes are associated with active histone marks and enriched with 5-hydroxymethylcytosine sites. In hESCs, genes within the interactomes have elevated expression. Additionally, some genes associated with the POU5F1 enhancer contribute to pluripotency. Binding sites for multiple DNA binding proteins, including ATF3, CTCF, GABPA, JUND, NANOG, RAD21 and YY1, are enriched in both interactomes. The RARG locus, frequently interacting with the POU5F1 locus, has abundant RAD21 binding sites co-localized with other protein binding sites. Thus the interactomes of these two pluripotency genes could be an important part of the regulatory network in hESCs.
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