SynthesisBMC neuroscience2013
Method parameters' impact on mortality and variability in rat stroke experiments: a meta-analysis.
Synthesis in BMC neuroscience, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers, 4 of them syntheses that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed, 4 syntheses or guidelines pooled it, 53 citations in OpenAlex.
- Ginsenoside-Rb1 for Ischemic Stroke: A Systematic Review and Meta-analysis of Preclinical Evidence and Possible Mechanisms.Frontiers in pharmacology · 2020Pooled it
- Pooled it
- Efficacy of Alteplase in a Mouse Model of Acute Ischemic Stroke: A Retrospective Pooled Analysis.Stroke · 2016Pooled it
- Method parameters' impact on mortality and variability in mouse stroke experiments: a meta-analysis.Scientific reports · 2016Pooled it
- Periorbital Placement of a Laser Doppler Probe for Cerebral Blood Flow Monitoring Prior to Middle Cerebral Artery Occlusion in Rodent Models.Journal of visualized experiments : JoVE · 2024Article
- What can the common fruit fly teach us about stroke?: lessons learned from the hypoxic tolerantFrontiers in cellular neuroscience · 2024Review
- Post-reperfusion acute MR diffusion in stroke is a potential predictor for clinical outcome in rats.Scientific reports · 2023Article
- Preclinical Stroke Research and Translational Failure: A Bird's Eye View on Preventable Variables.Cellular and molecular neurobiology · 2022Review
- Hemodynamics and Tissue Optical Properties in Bimodal Infarctions Induced by Middle Cerebral Artery Occlusion.International journal of molecular sciences · 2022Article
- Optimisation of a Mouse Model of Cerebral Ischemia-Reperfusion to Address Issues of Survival and Model Reproducibility and Consistency.Computational intelligence and neuroscience · 2022Article
- Meta-analysis of variation suggests that embracing variability improves both replicability and generalizability in preclinical research.PLoS biology · 2021Article
- Metabolome Changes in Cerebral Ischemia.Cells · 2020Review
- Direct myosin-2 inhibition enhances cerebral perfusion resulting in functional improvement after ischemic stroke.Theranostics · 2020Article
- Effect of laser Doppler flowmetry and occlusion time on outcome variability and mortality in rat middle cerebral artery occlusion: inconclusive results.BMC neuroscience · 2018Article
- Danhong Injection Reversed Cardiac Abnormality in Brain-Heart Syndrome via Local and Remote β-Adrenergic Receptor Signaling.Frontiers in pharmacology · 2018Article
- Triple-Gene Therapy for Stroke: A Proof-of-ConceptFrontiers in pharmacology · 2018Article
- Effects of high and low 17β-estradiol doses on focal cerebral ischemia in rats.Scientific reports · 2016Article
- Cerebral collateral circulation in experimental ischemic stroke.Experimental & translational stroke medicine · 2016Review
- Animal models of ischemic stroke and their application in clinical research.Drug design, development and therapy · 2015Review
- Clot injection technique affects thrombolytic efficacy in a rat embolic stroke model: implications for translaboratory collaborations.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundEven though more than 600 stroke treatments have been shown effective in preclinical studies, clinically proven treatment alternatives for cerebral infarction remain scarce. Amongst the reasons for the discrepancy may be methodological shortcomings, such as high mortality and outcome variability, in the preclinical studies. A common approach in animal stroke experiments is that A) focal cerebral ischemia is inflicted, B) some type of treatment is administered and C) the infarct sizes are assessed. However, within this paradigm, the researcher has to make numerous methodological decisions, including choosing rat strain and type of surgical procedure. Even though a few studies have attempted to address the questions experimentally, a lack of consensus regarding the optimal methodology remains.
methodsWe therefore meta-analyzed data from 502 control groups described in 346 articles to find out how rat strain, procedure for causing focal cerebral ischemia and the type of filament coating affected mortality and infarct size variability.
resultsThe Wistar strain and intraluminal filament procedure using a silicone coated filament was found optimal in lowering infarct size variability. The direct and endothelin methods rendered lower mortality rate, whereas the embolus method increased it compared to the filament method.
conclusionsThe current article provides means for researchers to adjust their middle cerebral artery occlusion (MCAo) protocols to minimize infarct size variability and mortality.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.