ArticleBMC developmental biology2013
Evidence for the requirement of 14-3-3eta (YWHAH) in meiotic spindle assembly during mouse oocyte maturation.
Article in BMC developmental biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 32 citations in OpenAlex.
- The subcortical maternal complex modulates the cell cycle during early mammalian embryogenesis via 14-3-3.Nature communications · 2024Article
- The phospho-docking protein 14-3-3 regulates microtubule-associated proteins in oocytes including the chromosomal passenger Borealin.PLoS genetics · 2022Article
- Maternal Smc3 protects the integrity of the zygotic genome through DNA replication and mitosis.Development (Cambridge, England) · 2021Article
- Circ_0015756 promotes the progression of ovarian cancer by regulating miR-942-5p/CUL4B pathway.Cancer cell international · 2020Article
- METTL3-mediated mCell cycle (Georgetown, Tex.) · 2020Article
- Identification of PKP 2/3 as potential biomarkers of ovarian cancer based on bioinformatics and experiments.Cancer cell international · 2020Article
- YWHA (14-3-3) protein isoforms and their interactions with CDC25B phosphatase in mouse oogenesis and oocyte maturation.BMC developmental biology · 2019Article
- Junctional tumor suppressors interact with 14-3-3 proteins to control planar spindle alignment.The Journal of cell biology · 2019Article
- Differential abundance and transcription of 14-3-3 proteins during vegetative growth and sexual reproduction in budding yeast.Scientific reports · 2018Article
- Recent advances in understanding oogenesis: interactions with the cytoskeleton, microtubule organization, and meiotic spindle assembly in oocytes.F1000Research · 2018Review
- 14-3-3 regulation of Ncd reveals a new mechanism for targeting proteins to the spindle in oocytes.The Journal of cell biology · 2017Article
- iTRAQ-Based Quantitative Proteomic Analysis of the Inhibitory Effects of Polysaccharides from Viscum coloratum (Kom.) Nakai on HepG2 Cells.Scientific reports · 2017Article
- GADD45β, an anti-tumor gene, inhibits avian leukosis virus subgroup J replication in chickens.Oncotarget · 2016Article
- NPR2 is involved in FSH-mediated mouse oocyte meiotic resumption.Journal of ovarian research · 2016Article
- microRNA-320/RUNX2 axis regulates adipocytic differentiation of human mesenchymal (skeletal) stem cells.Cell death & disease · 2014Article
- 14-3-3 epsilon prevents G2/M transition of fertilized mouse eggs by binding with CDC25B.BMC developmental biology · 2014Article
- A NudE/14-3-3 pathway coordinates dynein and the kinesin Khc73 to position the mitotic spindle.Developmental cell · 2013Article
Corrections and comments
- Erratum issued
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
backgroundThe 14-3-3 (YWHA) proteins are central mediators in various cellular signaling pathways regulating development and growth, including cell cycle regulation. We previously reported that all seven mammalian 14-3-3 isoforms are expressed in mouse oocytes and eggs and that, 14-3-3η (YWHAH) accumulates and co-localizes in the region of meiotic spindle in mouse eggs matured in vivo. Therefore, we investigated the role of 14-3-3η in spindle formation during mouse oocyte maturation.
resultsExamination of oocytes matured in vitro demonstrated that 14-3-3η accumulates in both meiosis I and II spindles. To explore if 14-3-3η interacts directly with α-tubulin in meiotic spindles, we performed an in situ proximity ligation assay that can detect intracellular protein-protein interactions at the single molecule level and which allows visualization of the actual interaction sites. This assay revealed a marked interaction between 14-3-3η and α-tubulin at the metaphase II spindle. To demonstrate a functional role for 14-3-3η in oocyte maturation, mouse oocytes were microinjected with a translation-blocking morpholino oligonucleotide against 14-3-3η mRNA to reduce 14-3-3η protein synthesis during oocyte maturation. Meiotic spindles in those cells were examined by immunofluorescence staining of 14-3-3η and α-tubulin along with observation of DNA. In 76% of cells injected with the morpholino, meiotic spindles were found to be deformed or absent and there was reduced or no accumulation of 14-3-3η in the spindle region. Those cells contained clumped chromosomes, with no polar body formation. Immunofluorescence staining of 14-3-3η and α-tubulin in control eggs matured in vitro from uninjected oocytes and oocytes microinjected with the ineffective, inverted form of a morpholino against 14-3-3η, a morpholino against 14-3-3γ, or deionized water showed normal, bipolar spindles.
conclusionsThe results indicate that 14-3-3η is essential for normal meiotic spindle formation during in vitro maturation of mouse oocytes, in part by interacting with α-tubulin, to regulate the assembly of microtubules. These data add to our understanding of the roles of 14-3-3 proteins in mouse oocyte maturation and mammalian reproduction.
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