Evidence map›Paper›PMID 23547015›Full record

ArticleCurrent protocols in molecular biology2013

Synthesis and labeling of RNA in vitro.

Chao Huang, Yi-Tao Yu

Abstract read
In one paragraph

Article in Current protocols in molecular biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Chao HuangProcess Science Downstream, Bristol-Myers Squibb Company, East Syracuse, New York, USA.
Yi-Tao Yu

Funding

The University of Rochester's Clinical and Translational Science InstituteUL1TR000042 · NCATS · UNIVERSITY OF ROCHESTER · PI BENNETT, NANCY M, KIEBURTZ, KARL D. · 2012 to 2015
$14.4M
Expanding the genetic code by targeted pseudouridylationR01GM104077 · NIGMS · UNIVERSITY OF ROCHESTER · PI YU, YI-TAO · 2012 to 2015
$1.2M
Regulation of telomerase activity and aging by 2'-O-methylation in telomerase RNAR21AG039559 · NIA · UNIVERSITY OF ROCHESTER · PI YU, YI-TAO · 2012 to 2013
$412k
NCATS NIH HHS UL1 TR000042NCATS NIH HHS UL1TR000042NIA NIH HHS AG39559NIA NIH HHS R21 AG039559NIGMS NIH HHS GM104077NIGMS NIH HHS R01 GM104077
6 · The paper itself

Abstract

This unit discusses several methods for generating large amounts of uniformly labeled, end-labeled, and site-specifically labeled RNAs in vitro. The methods involve a number of experimental procedures, including RNA transcription, 5' dephosphorylation and rephosphorylation, 3' terminal nucleotide addition (via ligation), site-specific RNase H cleavage directed by 2'-O-methyl RNA-DNA chimeras, and 2-piece splint ligation. The applications of these RNA radiolabeling approaches are also discussed.

Indexed as

RNAIn Vitro TechniquesRadioisotopesRibonuclease HStaining and LabelingSubstrate SpecificityTranscription, GeneticRadioisotopesRibonuclease HRNA

Identifiers

PMID23547015
PMCPMC3736555

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.