Evidence map›Paper›PMID 23546666›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2013

Single-cell nanosurgery.

Maxwell B Zeigler, Daniel T Chiu

Abstract read
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Maxwell B ZeiglerDepartment of Chemistry, University of Washington, Seattle, WA, USA.
Daniel T Chiu

Funding

High-Resolution Analysis of Synaptic ProteinsR01NS052637 · NINDS · UNIVERSITY OF WASHINGTON · PI CHIU, DANIEL T · 2005 to 2013
$3.2M
Single-Molecule Studies of Synaptic VesiclesR01NS062725 · NINDS · UNIVERSITY OF WASHINGTON · PI CHIU, DANIEL T · 2008 to 2011
$1.3M
Super-Resolution Imaging with Difference Deconvolution MicroscopyR01GM085485 · NIGMS · UNIVERSITY OF WASHINGTON · PI CHIU, DANIEL T · 2008 to 2011
$1.2M
NIGMS NIH HHS GM085485NIGMS NIH HHS R01 GM085485NINDS NIH HHS NS052637NINDS NIH HHS NS062725NINDS NIH HHS R01 NS052637NINDS NIH HHS R01 NS062725
6 · The paper itself

Abstract

This chapter explains the steps necessary to perform laser surgery upon single adherent mammalian cells, where individual organelles are extracted from the cells by optical tweezers and the cells are monitored post-surgery to check their viability. Single-cell laser nanosurgery is used in an increasing range of methodologies because it offers great flexibility. Its main advantages are (a) there is not any physical contact with the cells so they remain in a sterile environment, (b) high spatial selectivity so that single organelles can be extracted from specific areas of individual cells, (c) the method can be conducted in the cell's native media, and (d) in comparison to other techniques that target single cells, such as micromanipulators, laser nanosurgery has a comparatively high throughput.

Indexed as

NanotechnologyAnimalsCHO CellsCricetinaeCricetulusLaser TherapyOptical Tweezers

Identifiers

PMID23546666
PMCPMC5731472

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.