ArticleCurrent protocols in protein science2013
Preparation of recombinant protein spotted arrays for proteome-wide identification of kinase targets.
Article in Current protocols in protein science, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
3 citing papers in PubMed.
- Effect of sonication and protease inhibitors on Elisa quantification of selected proteins in bovine udder tissue homogenates.Scientific reports · 2026Article
- Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions.Journal of visualized experiments : JoVE · 2015Article
- Protein microarrays: a new tool for the study of autoantibodies in immunodeficiency.Frontiers in immunology · 2015Review
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2 authors.
Funding
Abstract
Protein microarrays allow unique approaches for interrogating global protein interaction networks. Protein arrays can be divided into two categories: antibody arrays and functional protein arrays. Antibody arrays consist of various antibodies and are appropriate for profiling protein abundance and modifications. Functional full-length protein arrays employ full-length proteins with various post-translational modifications. A key advantage of the latter is rapid parallel processing of large number of proteins for studying highly controlled biochemical activities, protein-protein interactions, protein-nucleic acid interactions, and protein-small molecule interactions. This unit presents a protocol for constructing functional yeast protein microarrays for global kinase substrate identification. This approach enables the rapid determination of protein interaction networks in yeast on a proteome-wide level. The same methodology can be readily applied to higher eukaryotic systems with careful consideration of overexpression strategy.
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