ArticleCurrent protocols in protein science2013
Overview of electron crystallography of membrane proteins: crystallization and screening strategies using negative stain electron microscopy.
Article in Current protocols in protein science, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 33 citations in OpenAlex.
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- Hallucinating symmetric protein assemblies.Science (New York, N.Y.) · 2022Article
- Computational design of mechanically coupled axle-rotor protein assemblies.Science (New York, N.Y.) · 2022Article
- Studying membrane proteins with MicroED.Biochemical Society transactions · 2022Article
- Reconfigurable asymmetric protein assemblies through implicit negative design.Science (New York, N.Y.) · 2022Article
- Protein Assembly by Design.Chemical reviews · 2021Review
- Microbial production of megadalton titin yields fibers with advantageous mechanical properties.Nature communications · 2021Article
- Design and structure of two new protein cages illustrate successes and ongoing challenges in protein engineering.Protein science : a publication of the Protein Society · 2020Article
- Covalently-assembled single-chain protein nanostructures with ultra-high stability.Nature communications · 2019Article
- The cryo-EM method microcrystal electron diffraction (MicroED).Nature methods · 2019Review
- From electron crystallography of 2D crystals to MicroED of 3D crystals.Current opinion in colloid & interface science · 2018Article
- Article
- Article
- MicroED opens a new era for biological structure determination.Current opinion in structural biology · 2016Review
- Accurate design of megadalton-scale two-component icosahedral protein complexes.Science (New York, N.Y.) · 2016Article
- Second harmonic generation correlation spectroscopy for characterizing translationally diffusing protein nanocrystals.Acta crystallographica. Section D, Structural biology · 2016Article
- The collection of MicroED data for macromolecular crystallography.Nature protocols · 2016Article
- Protein structure determination by MicroED.Current opinion in structural biology · 2014Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Electron cryomicroscopy, or cryoEM, is an emerging technique for studying the three-dimensional structures of proteins and large macromolecular machines. Electron crystallography is a branch of cryoEM in which structures of proteins can be studied at resolutions that rival those achieved by X-ray crystallography. Electron crystallography employs two-dimensional crystals of a membrane protein embedded within a lipid bilayer. The key to a successful electron crystallographic experiment is the crystallization, or reconstitution, of the protein of interest. This unit describes ways in which protein can be expressed, purified, and reconstituted into well-ordered two-dimensional crystals. A protocol is also provided for negative stain electron microscopy as a tool for screening crystallization trials. When large and well-ordered crystals are obtained, the structures of both protein and its surrounding membrane can be determined to atomic resolution.
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