ArticlePloS one2013
Doxycycline-regulated 3T3-L1 preadipocyte cell line with inducible, stable expression of adenoviral E4orf1 gene: a cell model to study insulin-independent glucose disposal.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- E4orf1 Prevents Progression of Fatty Liver Disease in Mice on High Fat Diet.International journal of molecular sciences · 2022Article
- Effects of Isorhamnetin on Diabetes and Its Associated Complications: A Review of In Vitro and In Vivo Studies and a Post Hoc Transcriptome Analysis of Involved Molecular Pathways.International journal of molecular sciences · 2022Review
- Long-term infection passaging of Human Adenovirus 36 in monkey kidney cells.Revista do Instituto de Medicina Tropical de Sao Paulo · 2022Article
- Nanoparticle-mediated in vitro delivery of E4orf1 to preadipocytes is a clinically relevant delivery system to improve glucose uptake.International journal of obesity (2005) · 2020Article
- Progesterone receptor isoform B expression in pulmonary neuroendocrine cells decreases cell proliferation.The Journal of steroid biochemistry and molecular biology · 2019Article
- E4orf1 protein reduces the need for endogenous insulin.Nutrition & diabetes · 2019Article
- E4orf1: A protein for enhancing glucose uptake despite impaired proximal insulin signaling.PloS one · 2018Article
- Hepatic Expression of Adenovirus 36 E4ORF1 Improves Glycemic Control and Promotes Glucose Metabolism Through AKT Activation.Diabetes · 2017Article
- Progesterone receptor (PR) polyproline domain (PPD) mediates inhibition of epidermal growth factor receptor (EGFR) signaling in non-small cell lung cancer cells.Cancer letters · 2016Article
- Adenovirus 36 DNA in human adipose tissue.International journal of obesity (2005) · 2015Article
- E4orf1 induction in adipose tissue promotes insulin-independent signaling in the adipocyte.Molecular metabolism · 2015Article
- Adenovirus 36 and Obesity: An Overview.Viruses · 2015Review
- ADV36 adipogenic adenovirus in human liver disease.World journal of gastroenterology · 2014Review
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Impaired glycemic control and excessive adiposity are major risk factors for Type 2 Diabetes mellitus. In rodent models, Ad36, a human adenovirus, improves glycemic control, independent of dietary fat intake or adiposity. It is impractical to use Ad36 for therapeutic action. Instead, we identified that E4orf1 protein of Ad36, mediates its anti-hyperglycemic action independent of insulin signaling. To further evaluate the therapeutic potential of E4orf1 to improve glycemic control, we established a stable 3T3-L1 cell system in which E4orf1 expression can be regulated. The development and characterization of this cell line is described here. Full-length adenoviral-36 E4orf1 cDNA obtained by PCR was cloned into a tetracycline responsive element containing vector (pTRE-Tight-E4orf1). Upon screening dozens of pTRE-Tight-E4orf1 clones, we identified the one with the highest expression of E4orf1 in response to doxycycline treatment. Furthermore, using this inducible system we characterized the ability of E4orf1 to improve glucose disposal in a time dependent manner. This stable cell line offers a valuable resource to carefully study the novel signaling pathways E4orf1 uses to enhance cellular glucose disposal independent of insulin.
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