ArticlePloS one2013
Sperm cell population dynamics in ram semen during the cryopreservation process.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Sperm Recovery and Evaluation in Domestic Species: Challenges and Current Practices.Reproduction in domestic animals = Zuchthygiene · 2026Review
- Unraveling sperm kinematic heterogeneity with machine learning.Asian journal of andrology · 2026Review
- Effects of long-term cryopreservation on sperm quality and oxidative stress in Manchega rams (Ovis aries).Reproduction (Cambridge, England) · 2025Article
- Effect of Breed and Season in Buck Semen Cryopreservation: The Portuguese Animal Germplasm Bank.Veterinary sciences · 2024Article
- Impact of various cryo-preservation steps on sperm rheotaxis and sperm kinematics in bull.Scientific reports · 2024Article
- Application of Flow Cytometry Using Advanced Chromatin Analyses for Assessing Changes in Sperm Structure and DNA Integrity in a Porcine Model.International journal of molecular sciences · 2024Article
- Cryopreservation of ram semen: baicalein efficiency on oxidative stress, chromatin integrity, viability and motility post thaw.Frontiers in veterinary science · 2024Article
- Sperm quality and morphometry characterization of cryopreserved canine sperm in ACP-106c or TRIS.Animal reproduction · 2022Article
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- Centrifugal force assessment in ram sperm: identifying species-specific impact.Acta veterinaria Scandinavica · 2021Article
- ProAKAP4 as Novel Molecular Marker of Sperm Quality in Ram: An Integrative Study in Fresh, Cooled and Cryopreserved Sperm.Biomolecules · 2020Article
- Sperm Cryodamage in Ruminants: Understanding the Molecular Changes Induced by the Cryopreservation Process to Optimize Sperm Quality.International journal of molecular sciences · 2020Review
- Effects of the cryopreservation process on dog sperm integrity.Animal reproduction · 2020Article
- Improving sperm banking efficiency in endangered species through the use of a sperm selection method in brown bear (Ursus arctos) thawed sperm.BMC veterinary research · 2017Article
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- Article
- Current status and potential of morphometric sperm analysis.Asian journal of andrologyReview
Corrections and comments
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundSperm cryopreservation has become an indispensable tool in biology. Initially, studies were aimed towards the development of efficient freezing protocols in different species that would allow for an efficient storage of semen samples for long periods of time, ensuring its viability. Nowadays, it is widely known that an important individual component exists in the cryoresistance of semen, and efforts are aimed at identifying those sperm characteristics that may allow us to predict this cryoresistance. This knowledge would lead, ultimately, to the design of optimized freezing protocols for the sperm characteristics of each male. METHODOLOGY/PRINCIPAL
findingsWe have evaluated the changes that occur in the sperm head dimensions throughout the cryopreservation process. We have found three different patterns of response, each of one related to a different sperm quality at thawing. We have been able to characterize males based on these patterns. For each male, its pattern remained constant among different ejaculates. This latter would imply that males always respond in the same way to freezing, giving even more importance to this sperm feature. CONCLUSIONS/SIGNIFICANCE: Changes in the sperm head during cryopreservation process have resulted useful to identify the ability of semen of males for freezing. We suggest that analyses of these response patterns would represent an important tool to characterize the cryoresistance of males when implemented within breeding programs. We also propose follow-up experiments to examine the outcomes of the use of different freezing protocols depending on the pattern of response of males.
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