ArticleJournal of bacteriology2013
Relaxed cleavage specificity within the RelE toxin family.
Article in Journal of bacteriology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 44 citations in OpenAlex.
- Acquisition and erosion of toxin-antitoxin systems in bacterial chromosomes.Molecular biology and evolution · 2026Article
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- Evaluating the Contribution of the Predicted Toxin-Antitoxin System HigBA to Persistence, Biofilm Formation, and Virulence in Burkholderia pseudomallei.Infection and immunity · 2022Article
- Biology and evolution of bacterial toxin-antitoxin systems.Nature reviews. Microbiology · 2022Review
- Stress Can Induce Transcription of Toxin-Antitoxin Systems without Activating Toxin.Molecular cell · 2020Article
- The Variety in the Common Theme of Translation Inhibition by Type II Toxin-Antitoxin Systems.Frontiers in genetics · 2020Review
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- Towards Exploring Toxin-Antitoxin Systems inInternational journal of molecular sciences · 2019Article
- Article
- AtaT blocks translation initiation by N-acetylation of the initiator tRNANature chemical biology · 2017Article
- Cut in translation: ribosome-dependent mRNA decay.The EMBO journal · 2017Article
- Emerging Roles of Toxin-Antitoxin Modules in Bacterial Pathogenesis.Molecules (Basel, Switzerland) · 2016Review
- Wake me when it's over - Bacterial toxin-antitoxin proteins and induced dormancy.Experimental biology and medicine (Maywood, N.J.) · 2016Review
- Transition State Charge Stabilization and Acid-Base Catalysis of mRNA Cleavage by the Endoribonuclease RelE.Biochemistry · 2015Article
- The Mycobacterium tuberculosis relBE toxin:antitoxin genes are stress-responsive modules that regulate growth through translation inhibition.Journal of microbiology (Seoul, Korea) · 2015Article
- Regulatory crosstalk between type I and type II toxin-antitoxin systems in the human pathogen Enterococcus faecalis.RNA biology · 2015Article
- Initiation of mRNA decay in bacteria.Cellular and molecular life sciences : CMLS · 2014Review
- The HicA toxin from Burkholderia pseudomallei has a role in persister cell formation.The Biochemical journal · 2014Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bacterial type II toxin-antitoxin systems are widespread in bacteria. Among them, the RelE toxin family is one of the most abundant. The RelE(K-12) toxin of Escherichia coli K-12 represents the paradigm for this family and has been extensively studied, both in vivo and in vitro. RelE(K-12) is an endoribonuclease that cleaves mRNAs that are translated by the ribosome machinery as these transcripts enter the A site. Earlier in vivo reports showed that RelE(K-12) cleaves preferentially in the 5'-end coding region of the transcripts in a codon-independent manner. To investigate whether the molecular activity as well as the cleavage pattern are conserved within the members of this toxin family, RelE-like sequences were selected in Proteobacteria, Cyanobacteria, Actinobacteria, and Spirochaetes and tested in E. coli. Our results show that these RelE-like sequences are part of toxin-antitoxin gene pairs, and that they inhibit translation in E. coli by cleaving transcripts that are being translated. Primer extension analyses show that these toxins exhibit specific cleavage patterns in vivo, both in terms of frequency and location of cleavage sites. We did not observe codon-dependent cleavage but rather a trend to cleave upstream purines and between the second and third positions of codons, except for the actinobacterial toxin. Our results suggest that RelE-like toxins have evolved to rapidly and efficiently shut down translation in a large spectrum of bacterial species, which correlates with the observation that toxin-antitoxin systems are spreading by horizontal gene transfer.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.