ArticleJournal of bacteriology2013
Activity and regulation of various forms of CwlJ, SleB, and YpeB proteins in degrading cortex peptidoglycan of spores of Bacillus species in vitro and during spore germination.
Article in Journal of bacteriology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 53 citations in OpenAlex.
- Article
- YpeB stability affects germination possibly through delaying SleB activity inJournal of bacteriology · 2026Article
- Spore-Forming Probiotic-Embedded Biomaterials for Targeted Gut Microplastic Biodegradation.ACS pharmacology & translational science · 2026Review
- Article
- Structural and functional analysis ofMicrobiology (Reading, England) · 2025Article
- Recent progress in proteins regulating the germination ofJournal of bacteriology · 2025Review
- A Family of Spore Lipoproteins Stabilizes the Germination Apparatus by Altering Inner Spore Membrane Fluidity inJournal of bacteriology · 2023Article
- Changes in Envelope Structure and Cell-Cell Communication during Akinete Differentiation and Germination in Filamentous CyanobacteriumLife (Basel, Switzerland) · 2022Article
- Occurrence of genes encoding spore germination inMicrobial genomics · 2022Article
- Suboptimal Bacillus licheniformis and Bacillus weihenstephanensis Spore Incubation Conditions Increase Heterogeneity of Spore Outgrowth Time.Applied and environmental microbiology · 2020Article
- SwsB and SafA Are Required for CwlJ-Dependent Spore Germination inJournal of bacteriology · 2020Article
- Sporulation and Germination in Clostridial Pathogens.Microbiology spectrum · 2019Review
- YpeB dimerization may be required to stabilize SleB for effective germination of Bacillus anthracis spores.BMC microbiology · 2019Article
- Structural and functional analyses of the N-terminal domain of the A subunit of aProceedings of the National Academy of Sciences of the United States of America · 2019Article
- Quantification and isolation of Bacillus subtilis spores using cell sorting and automated gating.PloS one · 2019Article
- Building of Pressure-Assisted Ultra-High Temperature System and Its Inactivation of Bacterial Spores.Frontiers in microbiology · 2019Article
- Stoichiometry, Absolute Abundance, and Localization of Proteins in the Bacillus cereus Spore Coat Insoluble Fraction Determined Using a QconCAT Approach.Journal of proteome research · 2018Article
- Lytic transglycosylases: concinnity in concision of the bacterial cell wall.Critical reviews in biochemistry and molecular biology · 2017Review
- Analysis of the Germination of IndividualFrontiers in microbiology · 2017Article
- Surviving Between Hosts: Sporulation and Transmission.Microbiology spectrum · 2016Review
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
Abstract
Germination of Bacillus spores requires degradation of a modified layer of peptidoglycan (PG) termed the spore cortex by two redundant cortex-lytic enzymes (CLEs), CwlJ and SleB, plus SleB's partner protein, YpeB. In this study, in vitro and in vivo analyses have been used to clarify the roles of individual SleB and YpeB domains in PG degradation. Purified mature Bacillus cereus SleB without its signal sequence (SleB(M)) and the SleB C-terminal catalytic domain (SleB(C)) efficiently triggered germination of decoated Bacillus megaterium and Bacillus subtilis spores lacking endogenous CLEs; previously, SleB's N-terminal domain (SleB(N)) was shown to bind PG but have no enzymatic activity. YpeB lacking its putative membrane anchoring sequence (YpeB(M)) or its N- and C-terminal domains (YpeB(N) and YpeB(C)) alone did not exhibit degradative activity, but YpeB(N) inhibited SleB(M) and SleB(C) activity in vitro. The severe germination defect of B. subtilis cwlJ sleB or cwlJ sleB ypeB spores was complemented by ectopic expression of full-length sleB [sleB(FL)] and ypeB [ypeB(FL)], but normal levels of SleB(FL) in spores required normal spore levels of YpeB(FL) and vice versa. sleB(FL) or ypeB(FL) alone, sleB(FL) plus ypeB(C) or ypeB(N), and sleB(C) or sleB(N) plus ypeB(FL) did not complement the cortex degradation defect in cwlJ sleB ypeB spores. In addition, ectopic expression of sleB(FL) or cwlJ(FL) with a Glu-to-Gln mutation in a predicted active-site residue failed to restore the germination of cwlJ sleB spores, supporting the role of this invariant glutamate as the key catalytic residue in SleB and CwlJ.
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