ArticlePlant cell reports2013
Wuschel overexpression promotes somatic embryogenesis and induces organogenesis in cotton (Gossypium hirsutum L.) tissues cultured in vitro.
Article in Plant cell reports, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 76 papers.
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Who cites it
76 citing papers in PubMed.
- Boosting genome editing in perennial plants by CRISPR-Combo mediated morphogenic gene activation.Nature communications · 2026Article
- The AT-HOOK MOTIF CONTAINING NUCLEAR LOCALIZED gene promotes somatic embryogenesis and polyploidy formation in Liriodendron hybrids.Plant cell reports · 2026Article
- WUSCHEL in forest trees: evolutionary organizer, vascular architect, and totipotency switch.Plant molecular biology · 2026Review
- Genome-wide identification and expression analysis of the maize ZmWOX gene family reveal its critical role in callus formation.BMC genomics · 2026Article
- Chromosome-level genome assembly, annotation, evolutionary analysis, and establishment of transformation systems in Carex rigescens.Plant cell reports · 2026Article
- WUSCHEL Transcription Factor: From Stem Cell Maintenance to Crop Improvement.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- Advances in the use of morphogenic regulators and peptide regenerating factors for boosting plant transformation and genome editing.Frontiers in plant science · 2026Review
- TheForestry research · 2026Article
- Morphogenetic Factors as a Tool for Enhancing Plant Regeneration Capacity During In Vitro Transformation.International journal of molecular sciences · 2025Review
- Efficient and versatile rapeseed transformation for new breeding technologies.The Plant journal : for cell and molecular biology · 2025Article
- Arabidopsis as a model for translational research.The Plant cell · 2025Review
- Trichostatin A promotes de novo shoot regeneration from Arabidopsis root explants via a cytokinin related pathway.Scientific reports · 2025Article
- Advances in doubled haploid technology for rice breeding: mechanisms, applications, and future perspectives.Frontiers in plant science · 2025Review
- Developmental regulatory genes in recalcitrant forest trees: advances in somatic regeneration and genetic transformation.Frontiers in plant science · 2025Review
- Article
- The Identification and Characterization of WOX Family Genes inInternational journal of molecular sciences · 2024Article
- Overexpression of Vitis GRF4-GIF1 improves regeneration efficiency in diploid Fragaria vesca Hawaii 4.Plant methods · 2024Article
- Functional Mechanisms and the Application of Developmental Regulators for Improving Genetic Transformation in Plants.Plants (Basel, Switzerland) · 2024Review
- Proteomic and Phosphoproteomic Analyses during Plant Regeneration Initiation in Cotton (Genes · 2024Article
- Small molecules, enormous functions: potential approach for overcoming bottlenecks in embryogenic tissue induction and maintenance in conifers.Horticulture research · 2024Review
16 more citing papers are in PubMed but not listed here.
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Authors and funding
7 authors.
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Abstract
key messageThis work shows that overexpression of the WUS gene from Arabidopsis enhanced the expression of embryogenic competence and triggered organogenesis from some cells of the regenerated embryo-like structures. Agrobacterium-mediated genetic transformation of cotton was described in the late 1980s, but is still time consuming and largely genotype dependant due to poor regeneration. To help solve this bottleneck, we over-expressed the WUSCHEL (WUS) gene, a homeobox transcription factor cloned in Arabidopsis thaliana, known to stimulate organogenesis and/or somatic embryogenesis in Arabidopsis tissues cultured in vitro. The AtWUS gene alone, and AtWUS gene fused to the GFP marker were compared to the GFP gene alone and to an empty construct used as a control. Somatic embryogenesis was improved in WUS expressed calli, as the percentage of explants giving rise to embryogenic tissues was significantly higher (×3) when WUS gene was over-expressed than in the control. An interesting result was that WUS embryogenic lines evolved in green embryo-like structures giving rise to ectopic organogenesis never observed in any of our previous transformation experiments. Using our standard in vitro culture protocol, the overexpression of AtWUS in tissues of a recalcitrant variety did not result in the production of regenerated plants. This achievement will still require the optimization of other non-genetic factors, such as the balance of exogenous phytohormones. However, our results suggest that targeted expression of the WUS gene is a promising strategy to improve gene transfer in recalcitrant cotton cultivars.
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