ArticlePlant molecular biology2013
RNAi silencing of three homologues of S-adenosylmethionine decarboxylase gene in tapetal tissue of tomato results in male sterility.
Article in Plant molecular biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 56 citations in OpenAlex.
- Genic male and female sterility in vegetable crops.Horticulture research · 2023Article
- RNA Interference and CRISPR/Cas Gene Editing for Crop Improvement: Paradigm Shift towards Sustainable Agriculture.Plants (Basel, Switzerland) · 2021Review
- Plant Transcriptome Reprograming and Bacterial Extracellular Metabolites Underlying Tomato Drought Resistance Triggered by a Beneficial Soil Bacteria.Metabolites · 2021Article
- B-class MADS-box TM6 is a candidate gene for tomato male sterile-15TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2019Article
- A putative bHLH transcription factor is a candidate gene forHorticulture research · 2019Article
- Nexus Between Spermidine and Floral Organ Identity and Fruit/Seed Set in Tomato.Frontiers in plant science · 2019Article
- RNAi-mediated silencing of spermidine synthase gene results in reduced reproductive potential in tobacco.Physiology and molecular biology of plants : an international journal of functional plant biology · 2018Article
- Polyamines in the life of Arabidopsis: profiling the expression of S-adenosylmethionine decarboxylase (SAMDC) gene family during its life cycle.BMC plant biology · 2017Article
- Transcriptome response and developmental implications of RNAi-mediated ODC knockdown in tobacco.Functional & integrative genomics · 2017Article
- Molecular cloning and characterization ofPhysiology and molecular biology of plants : an international journal of functional plant biology · 2017Article
- Polyamines in Pollen: From Microsporogenesis to Fertilization.Frontiers in plant science · 2016Review
- Transcript Analysis and Regulative Events during Flower Development in Olive (Olea europaea L.).PloS one · 2016Article
- Over-expression of mouse ornithine decarboxylase gene under the control of fruit-specific promoter enhances fruit quality in tomato.Plant molecular biology · 2015Article
- Ensuring Reproduction at High Temperatures: The Heat Stress Response during Anther and Pollen Development.Plants (Basel, Switzerland) · 2013Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Polyamines play very important role in various cellular metabolic functions, including floral induction, floral differentiation and fertility regulation. In the present study, S-adenosylmethionine decarboxylase (SAMDC), a key gene involved in polyamine biosynthesis, has been targeted in tapetal tissue of tomato using RNAi to examine its effect on tapetum development and pollen viability. The target SAMDC gene fragments of three homologues were cloned in a hairpin RNA construct under the control of tapetal-specific A9 promoter, which was used to generate several RNAi tomato plants. These RNAi lines expressed the intended small interfering RNAs in the anther and showed the aborted and sterile pollen exhibiting shrunken and distorted morphology. These RNAi tomato plants having sterile pollen, failed to set fruits but female fertility of the plants remained unaffected as cross pollination resulted in fruit setting. Expression profiling of SAMDC genes showed considerable decrease in transcripts of SAMDC1 (5-8 fold) and SAMDC2 and SAMDC3 (2-3 fold) in the anthers of RNAi plants. The other polyamine biosynthesis genes, ADC and SPDSYN exhibited ~1.5 fold decrease in their transcript levels. Presence of siRNA molecules specific to SAMDC homologues in anther and tapetal-specific activity of A9 promoter as shown with GUS reporter system of RNAi plants suggested down-regulation of the target genes in tapetum by RNAi. These observations indicate the importance of SAMDC, in turn polyamines in pollen development, and thus tapetum-specific down-regulation of SAMDC genes using RNAi can be used for developing male sterile plants.
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