ArticleTransgenic research2013
A threshold level of oxalate oxidase transgene expression reduces Cryphonectria parasitica-induced necrosis in a transgenic American chestnut (Castanea dentata) leaf bioassay.
Article in Transgenic research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
22 citing papers in PubMed, 107 citations in OpenAlex.
- Perspectives of Gene Editing for the Conservation of Plant Genetic Resources.Methods in molecular biology (Clifton, N.J.) · 2026Review
- Speed Breeding Transgenic American Chestnut Trees Toward Restoration.Plant direct · 2025Article
- A genome-guided strategy for climate resilience in American chestnut restoration populations.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Applying molecular and genetic methods to trees and their fungal communities.Applied microbiology and biotechnology · 2023Review
- Transcriptome analysis reveals key genes involved in the resistance to Cryphonectria parasitica during early disease development in Chinese chestnut.BMC plant biology · 2023Article
- Pathogen-induced expression of a blight tolerance transgene in American chestnut.Molecular plant pathology · 2022Article
- European and American chestnuts: An overview of the main threats and control efforts.Frontiers in plant science · 2022Review
- Bumble bee (Bombus impatiens) survival, pollen usage, and reproduction are not affected by oxalate oxidase at realistic concentrations in American chestnut (Castanea dentata) pollen.Transgenic research · 2021Article
- Genomic Approaches for Conservation Management in Australia under Climate Change.Life (Basel, Switzerland) · 2021Review
- Identification of Susceptibility Genes inPlants (Basel, Switzerland) · 2021Article
- Efficient Transformation of Somatic Embryos and Regeneration of Cork Oak Plantlets with A Gene (International journal of molecular sciences · 2021Article
- Wild Soybean Oxalyl-CoA Synthetase Degrades Oxalate and Affects the Tolerance to Cadmium and Aluminum Stresses.International journal of molecular sciences · 2020Article
- Spatiotemporal Controllability and Environmental Risk Assessment of Genetically Engineered Gene Drive Organisms from the Perspective of European Union Genetically Modified Organism Regulation.Integrated environmental assessment and management · 2020Review
- The Apoplast: A Key Player in Plant Survival.Antioxidants (Basel, Switzerland) · 2020Review
- Review
- Optimizing genomic selection for blight resistance in American chestnut backcross populations: A trade-off with American chestnut ancestry implies resistance is polygenic.Evolutionary applications · 2020Article
- Developing Blight-Tolerant American Chestnut Trees.Cold Spring Harbor perspectives in biology · 2019Review
- Expression Analysis of Oxalate Metabolic Pathway Genes Reveals Oxalate Regulation Patterns in Spinach.Molecules (Basel, Switzerland) · 2018Article
- Article
- Phenotypic Expression and Stability in a Large-Scale Field Study of Genetically Engineered Poplars Containing Sexual Containment Transgenes.Frontiers in bioengineering and biotechnology · 2018Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
American chestnut (Castanea dentata) was transformed with a wheat oxalate oxidase (oxo) gene in an effort to degrade the oxalic acid (OA) secreted by the fungus Cryphonectria parasitica, thus decreasing its virulence. Expression of OxO was examined under two promoters: a strong constitutive promoter, CaMV 35S, and a predominantly vascular promoter, VspB. Oxo gene transcription was quantified by RT-qPCR. Relative expression of OxO varied approximately 200 fold among events produced with the 35S-OxO. The lowest 35S-OxO event expressed approximately 3,000 fold higher than the highest VspB-OxO event. This was potentially due to the tissue-specific nature of the VspB-controlled expression, the strength of the CaMV 35S constitutive promoter, or position effects. Leaf assays measuring necrotic lesion length were conducted to better understand the relationship between OxO expression level and the blight fungus in planta. A threshold response was observed between the OxO expression level and the C. parasitica lesion length. Five events of the 35S-OxO line showed significantly reduced lesion length compared to the blight-susceptible American chestnut. More importantly, the lesion length in these five events was reduced to the same level as the blight-resistant Chinese chestnut, C. mollissima. This is the first report on enhanced pathogen resistance in transgenic American chestnut.
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