ArticleApplied and environmental microbiology2013
Identification and expression of genes involved in the conversion of daidzein and genistein by the equol-forming bacterium Slackia isoflavoniconvertens.
Article in Applied and environmental microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 61 papers, 1 of them a synthesis that pooled it.
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Who cites it
61 citing papers in PubMed, 1 synthesis or guideline pooled it, 119 citations in OpenAlex.
- Systematic review of the effects of the intestinal microbiota on selected nutrients and non-nutrients.European journal of nutrition · 2018Pooled it
- Dietary Polyphenols and Cardiovascular Health: From Bioavailability to Clinical Relevance.Molecules (Basel, Switzerland) · 2026Review
- Review
- How Gut Microbes Transform Dietary Phytonutrients: Enzymatic Pathways and Human Metabolite Exposure.Molecules (Basel, Switzerland) · 2026Review
- Reducing Antibiotic Dependence in Poultry: The Potential of Phytochemicals as Antibiotic Alternatives Against Bacterial Foodborne Pathogens.Tropical medicine and infectious disease · 2026Review
- A Whole-Cell Catalytic System for Equol Production Based on Daidzein Reductase Engineering.Molecules (Basel, Switzerland) · 2026Article
- Soy and the gut microbiome: a bi-directional relationship shaping nutrition and health.European journal of nutrition · 2026Review
- ColiFormer: A Transformer-Based Codon Optimization Model Balancing Multiple Objectives for EnhancedBioengineering (Basel, Switzerland) · 2026Article
- The Translational Coupling of Daidzein Reductase and Dihydrodaidzein Racemase Genes Improves the Production of Equol and Its Analogous Derivatives in Engineered Lactic Acid Bacteria.ACS synthetic biology · 2025Article
- Glycitin in Soy Beans: A Mine of Their Structures, Functions, and Potential Applications.Foods (Basel, Switzerland) · 2025Review
- S-equol status modulates skin response to soy isoflavones in postmenopausal women: results from a randomized placebo-controlled pilot trial.Frontiers in nutrition · 2025Article
- Landscape of flavonoid metabolism in human gut microbiome and its association with health and disease.Gut microbes reports · 2025Article
- Causal effect of gut microbiota on venous thromboembolism: a two-sample mendelian randomization study.Thrombosis journal · 2024Article
- Iron-sulfur cluster-dependent enzymes and molybdenum-dependent reductases in the anaerobic metabolism of human gut microbes.Metallomics : integrated biometal science · 2024Review
- Effect of Fermented Soy Beverage on Equol Production by Fecal Microbiota.Foods (Basel, Switzerland) · 2024Article
- Isoflavones Effects on Vascular and Endothelial Outcomes: How Is the Gut Microbiota Involved?Pharmaceuticals (Basel, Switzerland) · 2024Review
- HIV-associated neurocognitive disorder: key implications of the microbiota-gut-brain axis.Frontiers in microbiology · 2024Review
- Decreased Abundance of GenusGastro hep advances · 2024Article
- Association between microbiome and the development of adverse posttraumatic neuropsychiatric sequelae after traumatic stress exposure.Translational psychiatry · 2023Article
- Inhibition of TRPA1, Endoplasmic Reticulum Stress, Human Airway Epithelial Cell Damage, and EctopicPharmaceuticals (Basel, Switzerland) · 2023Article
1 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Gut bacteria play a key role in the metabolism of dietary isoflavones, thereby influencing the availability and bioactivation of these polyphenols in the intestine. The human intestinal bacterium Slackia isoflavoniconvertens converts the main soybean isoflavones daidzein and genistein to equol and 5-hydroxy-equol, respectively. Cell extracts of S. isoflavoniconvertens catalyzed the conversion of daidzein via dihydrodaidzein to equol and that of genistein to dihydrogenistein. Growth of S. isoflavoniconvertens in the presence of daidzein led to the induction of several proteins as observed by two-dimensional difference gel electrophoresis. Based on determined peptide sequences, we identified a cluster of eight genes encoding the daidzein-induced proteins. Heterologous expression of three of these genes in Escherichia coli and enzyme activity tests with the resulting cell extracts identified the corresponding gene products as a daidzein reductase (DZNR), a dihydrodaidzein reductase (DHDR), and a tetrahydrodaidzein reductase (THDR). The recombinant DZNR also converted genistein to dihydrogenistein at higher rates than were observed for the conversion of daidzein to dihydrodaidzein. Higher rates were also observed with cell extracts of S. isoflavoniconvertens. The recombinant DHDR and THDR catalyzed the reduction of dihydrodaidzein to equol, while the corresponding conversion of dihydrogenistein to 5-hydroxy-equol was not observed. The DZNR, DHDR, and THDR were expressed as Strep-tag fusion proteins and subsequently purified by affinity chromatography. The purified enzymes were further characterized with regard to their activity, stereochemistry, quaternary structure, and content of flavin cofactors.
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