ArticleApplied and environmental microbiology2013
Synthesis of fucosyl-N-acetylglucosamine disaccharides by transfucosylation using α-L-fucosidases from Lactobacillus casei.
Article in Applied and environmental microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 46 citations in OpenAlex.
- Transglycosylation Capabilities of Wild-Type α-l-Fucosidase iso1 from Paenibacillus thiaminolyticus and Its Engineered Mutants: Preparation of Fucosylated Oligosaccharides.Microbial biotechnology · 2026Article
- Exploring the sequence-function space of microbial fucosidases.Communications chemistry · 2024Article
- Synthesis of fucosyllactose using α-L-fucosidases GH29 from infant gut microbial metagenome.Applied microbiology and biotechnology · 2024Article
- α-L-Fucosidases from an Alpaca Faeces Metagenome: Characterisation of Hydrolytic and Transfucosylation Potential.International journal of molecular sciences · 2024Article
- Structure and function of microbial α-l-fucosidases: a mini review.Essays in biochemistry · 2023Review
- The dual role of fucosidases: tool or target.Biologia · 2023Review
- Characterization and stabilization of the α-L-fucosidase set from Lacticaseibacillus rhamnosus INIA P603.Applied microbiology and biotechnology · 2022Article
- Infant Gut Microbial Metagenome Mining of α-l-Fucosidases with Activity on Fucosylated Human Milk Oligosaccharides and Glycoconjugates.Microbiology spectrum · 2022Article
- Comparative studies on the substrate specificity and defucosylation activity of three α-l-fucosidases using synthetic fucosylated glycopeptides and glycoproteins as substrates.Bioorganic & medicinal chemistry · 2021Article
- An individual alginate lyase is effective in the disruption of Laminaria digitata recalcitrant cell wall.Scientific reports · 2021Article
- Human milk and mucosa-associated disaccharides impact on cultured infant fecal microbiota.Scientific reports · 2020Article
- Article
- Directed evolution of an α1,3-fucosyltransferase using a single-cell ultrahigh-throughput screening method.Science advances · 2019Article
- Synthesis of Fucosyl-Oligosaccharides Using α-l-Fucosidase fromMolecules (Basel, Switzerland) · 2019Article
- Site-selective chemoenzymatic glycoengineering of Fab and Fc glycans of a therapeutic antibody.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Improvement of the transfucosylation activity of α-L-fucosidase from Thermotoga maritima for the synthesis of fucosylated oligosaccharides in the presence of calcium and sodium.Extremophiles : life under extreme conditions · 2018Article
- Chemoenzymatic Defucosylation of Therapeutic Antibodies for Enhanced Effector Functions Using Bacterial α-Fucosidases.Methods in molecular biology (Clifton, N.J.) · 2018Article
- Purification of caprine oligosaccharides at pilot-scale.Journal of food engineering · 2017Article
- Designer α1,6-Fucosidase Mutants Enable Direct Core Fucosylation of Intact N-Glycopeptides and N-Glycoproteins.Journal of the American Chemical Society · 2017Article
- Epithelial glycosylation in gut homeostasis and inflammation.Nature immunology · 2016Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
AlfB and AlfC α-l-fucosidases from Lactobacillus casei were used in transglycosylation reactions, and they showed high efficiency in synthesizing fucosyldisaccharides. AlfB and AlfC activities exclusively produced fucosyl-α-1,3-N-acetylglucosamine and fucosyl-α-1,6-N-acetylglucosamine, respectively. The reaction kinetics showed that AlfB can convert 23% p-nitrophenyl-α-l-fucopyranoside into fucosyl-α-1,3-N-acetylglucosamine and AlfC at up to 56% into fucosyl-α-1,6-N-acetylglucosamine.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.