ArticleApplied and environmental microbiology2013
Biochemical diversity of carboxyl esterases and lipases from Lake Arreo (Spain): a metagenomic approach.
Article in Applied and environmental microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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19 citing papers in PubMed, 55 citations in OpenAlex.
- AMWEst, a new thermostable and detergent-tolerant esterase retrieved from the Albian aquifer.Applied microbiology and biotechnology · 2024Article
- The Mobility of the Cap Domain Is Essential for the Substrate Promiscuity of a Family IV Esterase from Sorghum Rhizosphere Microbiome.Applied and environmental microbiology · 2023Article
- Design of Artificial Enzymes Bearing Several Active Centers: New Trends, Opportunities and Problems.International journal of molecular sciences · 2022Review
- Metagenomic Mining for Esterases in the Microbial Community of Los Rueldos Acid Mine Drainage Formation.Frontiers in microbiology · 2022Article
- A promiscuous ancestral enzyme´s structure unveils protein variable regions of the highly diverse metallo-β-lactamase family.Communications biology · 2021Article
- Mechanism and Structural Insights Into a Novel Esterase, E53, Isolated FromFrontiers in microbiology · 2021Article
- Molecular and Biochemical Characterization of Salt-Tolerant Trehalose-6-Phosphate Hydrolases Identified by Screening and Sequencing Salt-Tolerant Clones From the Metagenomic Library of the Gastrointestinal Tract.Frontiers in microbiology · 2020Article
- Characterization of an acetyl xylan esterase from the marine bacterium Ochrovirga pacifica and its synergism with xylanase on beechwood xylan.Microbial cell factories · 2019Article
- Biotechnological potential of bacteria isolated from cattle environments of desert soils in Sonora Mexico.World journal of microbiology & biotechnology · 2018Article
- Aii810, a Novel Cold-AdaptedFrontiers in microbiology · 2017Article
- Characterization of a cold-active and salt tolerant esterase identified by functional screening of Arctic metagenomic libraries.BMC biochemistry · 2016Article
- Estimating the success of enzyme bioprospecting through metagenomics: current status and future trends.Microbial biotechnology · 2016Review
- Pre_GI: a global map of ontological links between horizontally transferred genomic islands in bacterial and archaeal genomes.Database : the journal of biological databases and curation · 2015Article
- Identification and characterization of carboxyl esterases of gill chamber-associated microbiota in the deep-sea shrimp Rimicaris exoculata by using functional metagenomics.Applied and environmental microbiology · 2015Article
- A combined bioinformatics and functional metagenomics approach to discovering lipolytic biocatalysts.Frontiers in microbiology · 2015Article
- Metagenomics of an Alkaline Hot Spring in Galicia (Spain): Microbial Diversity Analysis and Screening for Novel Lipolytic Enzymes.Frontiers in microbiology · 2015Article
- Synthetic biology approaches to improve biocatalyst identification in metagenomic library screening.Microbial biotechnology · 2015Review
- Biochemical studies on a versatile esterase that is most catalytically active with polyaromatic esters.Microbial biotechnology · 2014Article
- The production and characterization of a new active lipase from Acremonium alcalophilum using a plant bioreactor.Biotechnology for biofuels · 2013Article
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14 authors at 7 institutions in 3 countries.
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Abstract
The esterases and lipases from the α/β hydrolase superfamily exhibit an enormous sequence diversity, fold plasticity, and activities. Here, we present the comprehensive sequence and biochemical analyses of seven distinct esterases and lipases from the metagenome of Lake Arreo, an evaporite karstic lake in Spain (42°46'N, 2°59'W; altitude, 655 m). Together with oligonucleotide usage patterns and BLASTP analysis, our study of esterases/lipases mined from Lake Arreo suggests that its sediment contains moderately halophilic and cold-adapted proteobacteria containing DNA fragments of distantly related plasmids or chromosomal genomic islands of plasmid and phage origins. This metagenome encodes esterases/lipases with broad substrate profiles (tested over a set of 101 structurally diverse esters) and habitat-specific characteristics, as they exhibit maximal activity at alkaline pH (8.0 to 8.5) and temperature of 16 to 40°C, and they are stimulated (1.5 to 2.2 times) by chloride ions (0.1 to 1.2 M), reflecting an adaptation to environmental conditions. Our work provides further insights into the potential significance of the Lake Arreo esterases/lipases for biotechnology processes (i.e., production of enantiomers and sugar esters), because these enzymes are salt tolerant and are active at low temperatures and against a broad range of substrates. As an example, the ability of a single protein to hydrolyze triacylglycerols, (non)halogenated alkyl and aryl esters, cinnamoyl and carbohydrate esters, lactones, and chiral epoxides to a similar extent was demonstrated.
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