ArticleNature structural & molecular biology2013
Energetic role of the paddle motif in voltage gating of Shaker K(+) channels.
Article in Nature structural & molecular biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Dual mechanisms contribute to enhanced voltage dependence of an electric fish potassium channel.Biophysical journal · 2024Article
- Mapping temperature-dependent conformational change in the voltage-sensing domain of an engineered heat-activated KProceedings of the National Academy of Sciences of the United States of America · 2021Article
- Gating modules of the AMPA receptor pore domain revealed by unnatural amino acid mutagenesis.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Histone acetyltransferase CBP-related H3K23 acetylation contributes to courtship learning in Drosophila.BMC developmental biology · 2018Article
- Two distinct voltage-sensing domains control voltage sensitivity and kinetics of current activation in CaV1.1 calcium channels.The Journal of general physiology · 2016Article
- The Molecular Basis of Polyunsaturated Fatty Acid Interactions with the Shaker Voltage-Gated Potassium Channel.PLoS computational biology · 2016Article
- Equilibrium fluctuation relations for voltage coupling in membrane proteins.Biochimica et biophysica acta · 2015Article
- Voltage Sensing in Membranes: From Macroscopic Currents to Molecular Motions.The Journal of membrane biology · 2015Review
- Article
- Mechanosensitive gating of Kv channels.PloS one · 2015Review
- Article
- Coarse-grained simulations of the gating current in the voltage-activated Kv1.2 channel.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Divining the design principles of voltage sensors.The Journal of general physiology · 2014Article
- Evolutionary imprint of activation: the design principles of VSDs.The Journal of general physiology · 2014Article
- Animal toxins influence voltage-gated sodium channel function.Handbook of experimental pharmacology · 2014Review
- S3-S4 linker length modulates the relaxed state of a voltage-gated potassium channel.Biophysical journal · 2013Article
- Tuning voltage-gated channel activity and cellular excitability with a sphingomyelinase.The Journal of general physiology · 2013Article
- The design principle of paddle motifs in voltage sensors.Nature structural & molecular biology · 2013Article
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5 authors.
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Abstract
Voltage-gated ion channels underlie rapid electric signaling in excitable cells. Electrophysiological studies have established that the N-terminal half of the fourth transmembrane segment ((NT)S4) of these channels is the primary voltage sensor, whereas crystallographic studies have shown that (NT)S4 is not located within a proteinaceous pore. Rather, (NT)S4 and the C-terminal half of S3 ((CT)S3 or S3b) form a helix-turn-helix motif, termed the voltage-sensor paddle. This unexpected structural finding raises two fundamental questions: does the paddle motif also exist in voltage-gated channels in a biological membrane, and, if so, what is its function in voltage gating? Here, we provide evidence that the paddle motif exists in the open state of Drosophila Shaker voltage-gated K(+) channels expressed in Xenopus oocytes and that (CT)S3 acts as an extracellular hydrophobic 'stabilizer' for (NT)S4, thus biasing the gating chemical equilibrium toward the open state.
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