ArticleJournal of biomechanics2013
Dynamic mechanical compression of devitalized articular cartilage does not activate latent TGF-β.
Article in Journal of biomechanics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 28 citations in OpenAlex.
- Role of external forces in the mechanobiology of stem and differentiated chondrogenic cells embedded in a tissue-engineered construct for cartilage repair.Journal of biomedical science · 2026Review
- Latent TGF-β content of articular cartilage with development and age.Connective tissue research · 2026Article
- A latent TGF-β conjugated scaffold improves neocartilage development.Acta biomaterialia · 2026Article
- Physiologic Doses of Transforming Growth Factor-β Improve the Composition of Engineered Articular Cartilage.Tissue engineering. Part A · 2025Article
- Autoinduction-Based Quantification ofTissue engineering. Part C, Methods · 2024Article
- Advances in regenerative rehabilitation in the rehabilitation of musculoskeletal injuries.Regenerative medicine · 2024Review
- Computational and experimental characterizations of the spatiotemporal activity and functional role of TGF-β in the synovial joint.Journal of biomechanics · 2023Article
- Article
- Release of Matrix-Bound Growth Factors; the role of increase in Sodium Concentration with Cartilage Compression.Function (Oxford, England) · 2022Article
- Causal effects of inflammatory protein biomarkers on inflammatory diseases.Science advances · 2021Article
- Review
- Regenerative rehabilitation: The role of mechanotransduction in orthopaedic regenerative medicine.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2019Review
- Dissection of the molecular targets and signaling pathways of Guzhi Zengsheng Zhitongwan based on the analysis of serum proteomics.Chinese medicine · 2019Article
- Regenerative Rehabilitation of the Musculoskeletal System.The Journal of the American Academy of Orthopaedic Surgeons · 2018Article
- Differential Role of Transforming Growth Factor-beta in an Osteoarthritic or a Healthy Joint.Journal of bone metabolism · 2018Review
- [Intra-articular injection of ascorbic acid/ferric chloride relieves cartilage degradation in rats with osteoarthritis].Nan fang yi ke da xue xue bao = Journal of Southern Medical University · 2018Article
- Effect of Cyclic Dynamic Compressive Loading on Chondrocytes and Adipose-Derived Stem Cells Co-Cultured in Highly Elastic Cryogel Scaffolds.International journal of molecular sciences · 2018Article
- Article
- Heterogeneous engineered cartilage growth results from gradients of media-supplemented active TGF-β and is ameliorated by the alternative supplementation of latent TGF-β.Biomaterials · 2016Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
A growing body of research has highlighted the role that mechanical forces play in the activation of latent TGF-β in biological tissues. In synovial joints, it has recently been demonstrated that the mechanical shearing of synovial fluid, induced during joint motion, rapidly activates a large fraction of its soluble latent TGF-β content. Based on this observation, the primary hypothesis of the current study is that the mechanical deformation of articular cartilage, induced by dynamic joint motion, can similarly activate the large stores of latent TGF-β bound to the tissue extracellular matrix (ECM). Here, devitalized deep zone articular cartilage cylindrical explants (n=84) were subjected to continuous dynamic mechanical loading (low strain: ±2% or high strain: ±7.5% at 0.5Hz) for up to 15h or maintained unloaded. TGF-β activation was measured in these samples over time while accounting for the active TGF-β that remains bound to the cartilage ECM. Results indicate that TGF-β1 is present in cartilage at high levels (68.5±20.6ng/mL) and resides predominantly in the latent form (>98% of total). Under dynamic loading, active TGF-β1 levels did not statistically increase from the initial value nor the corresponding unloaded control values for any test, indicating that physiologic dynamic compression of cartilage is unable to directly activate ECM-bound latent TGF-β via purely mechanical pathways and leading us to reject the hypothesis of this study. These results suggest that deep zone articular chondrocytes must alternatively obtain access to active TGF-β through chemical-mediated activation and further suggest that mechanical deformation is unlikely to directly activate the ECM-bound latent TGF-β of various other tissues, such as muscle, ligament, and tendon.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.