ArticleAnalytica chimica acta2013
Practical considerations for preparing polymerized phospholipid bilayer capillary coatings for protein separations.
Article in Analytica chimica acta, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- Microfluidic-based electrically driven particle manipulation techniques for biomedical applications.RSC advances · 2025Review
- Review
- Highly stabilized, polymer-lipid membranes prepared on silica microparticles as stationary phases for capillary chromatography.Journal of chromatography. A · 2015Article
- Recent advances in the analysis of therapeutic proteins by capillary and microchip electrophoresis.Analytical methods : advancing methods and applications · 2014Article
- Hybrid phospholipid bilayer coatings for separations of cationic proteins in capillary zone electrophoresis.Electrophoresis · 2014Article
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5 authors.
Funding
Abstract
Phosphorylcholine (PC) based phospholipid bilayers have proven useful as capillary coating materials due to their inherent resistance to non-specific protein adsorption. The primary limitation of this important class of capillary coatings remains the limited long-term chemical and physical stability of the coatings. Recently, a method for increasing phospholipid coating stability in fused silica capillaries via utilization of polymerized, synthetic phospholipids was reported. Here, we expand upon these studies by investigating polymerized lipid bilayer capillary coatings with respect to separation performance including run-to-run, day-to-day and column-to-column reproducibility and long-term stability. In addition, the effects of pH and capillary inner diameter on polymerized phospholipid coated capillaries were investigated to identify optimized coating conditions. The coatings are stabilized for protein separations across a wide range of pH values (4.0-9.3), a unique property for capillary coating materials. Additionally, smaller inner diameter capillaries (≤50 μm) were found to yield marked enhancements in coating stability and reproducibility compared to wider bore capillaries, demonstrating the importance of capillary size for separations employing polymerized phospholipid coatings.
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Registered trials
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