ArticleJournal of clinical & experimental cardiology2012
Isolation, Characterization, and Spatial Distribution of Cardiac Progenitor Cells in the Sheep Heart.
Article in Journal of clinical & experimental cardiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
24 citing papers in PubMed, 31 citations in OpenAlex.
- Neonatally-derived multipotent Islet-1+ Mesp1+FOXA2+ stem cell clones restore cardiac function in sheep.Frontiers in cardiovascular medicine · 2025Article
- Expression of Stem Cell Niche-Related Biomarkers at the Base of the Human Tricuspid Valve.Stem cells and development · 2023Article
- Long-Term Hypoxia Maintains a State of Dedifferentiation and Enhanced Stemness in Fetal Cardiovascular Progenitor Cells.International journal of molecular sciences · 2021Article
- Review
- The Atrioventricular Junction: A Potential Niche Region for Progenitor Cells in the Adult Human Heart.Stem cells and development · 2019Article
- Effects of Spaceflight and Simulated Microgravity on YAP1 Expression in Cardiovascular Progenitors: Implications for Cell-Based Repair.International journal of molecular sciences · 2019Article
- Calcium-dependent potassium channels control proliferation of cardiac progenitor cells and bone marrow-derived mesenchymal stem cells.The Journal of physiology · 2018Article
- Short-term hypoxia improves early cardiac progenitor cell functionAmerican journal of stem cells · 2018Article
- Isolation, Characterization and Differentiation of Mouse Cardiac Progenitor Cells.Methods in molecular biology (Clifton, N.J.) · 2018Article
- Molecular basis of functional myogenic specification of Bona Fide multipotent adult cardiac stem cells.Cell cycle (Georgetown, Tex.) · 2018Review
- A Hyper-Crosslinked Carbohydrate Polymer Scaffold Facilitates Lineage Commitment and Maintains a Reserve Pool of Proliferating Cardiovascular Progenitors.Transplantation direct · 2017Article
- Cardiac Progenitor Cells and the Interplay with Their Microenvironment.Stem cells international · 2017Review
- An in vitro model for the assessment of stem cell fate following implantation within the infarct microenvironment identifies ISL-1 expression as the strongest predictor of c-Kit(+) cardiac progenitor cells' therapeutic potential.Journal of molecular and cellular cardiology · 2015Article
- Finding the rhythm of sudden cardiac death: new opportunities using induced pluripotent stem cell-derived cardiomyocytes.Circulation research · 2015Review
- c-kit(+) cells: the tell-tale heart of cardiac regeneration?Cellular and molecular life sciences : CMLS · 2015Review
- Cardiac regeneration using pluripotent stem cells--progression to large animal models.Stem cell research · 2014Review
- Isolation and characterization of resident endogenous c-Kit+ cardiac stem cells from the adult mouse and rat heart.Nature protocols · 2014Article
- Genes upregulated in prostate cancer reactive stroma promote prostate cancer progression in vivo.Clinical cancer research : an official journal of the American Association for Cancer Research · 2014Article
- OPN Polymorphism Is Related to the Chemotherapy Response and Prognosis in Advanced NSCLC.International journal of genomics · 2014Article
- BMP-4 genetic variants and protein expression are associated with platinum-based chemotherapy response and prognosis in NSCLC.BioMed research international · 2014Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
backgroundLaboratory large animal models are important for establishing the efficacy of stem cell therapies that may be translated into clinical use. The similarity of ovine and human cardiovascular systems provides an opportunity to use the sheep as a large animal model in which to optimize cell-based treatments for the heart. Recent clinical trials in humans using endogenous cardiovascular progenitor cells report significant improvement in cardiac function following stem cell-based therapy. To date, however, endogenous cardiovascular progenitor cells have not been isolated from the sheep heart.
methodsCardiovascular cells expressing SSEA-4, CD105 and c-kit were isolated by flow cytometry and cloned from the right atrium of neonatal sheep. The expression of GATA-4, c-kit, and Isl1 was identified by PCR in the cloned cells. Immunohistochemical staining was used to compare the number of SSEA-4 positive cells in the right auricle, right atrium, left ventricle and the apex of the heart of fetal, neonatal and adult sheep. The number of SSEA4+cells was also compared in fetal, pregnant and non-pregnant adult sheep.
resultsFour distinct cardiac progenitor cell sub-populations were identified in sheep, including CD105+SSEA-4+c-kit+Isl1+GATA-4+cells, CD105+SSEA-4+c-kit+Isl1+GATA-4-cells, CD105+SSEA-4-c-kit-Isl1+GATA-4-cells, and CD105+SSEA-4-c-kit+Isl1+GATA-4-cells. Immunohistochemical staining for SSEA-4 showed that labeled cells were most abundant in the right atrium of fetal hearts where niches of progenitor cells could be identified.
conclusionWe determined the phenotype and distribution of cardiac progenitor cells in the sheep heart. The availability of cloned endogenous cardiac progenitor cells from sheep will provide a valuable resource for optimizing the conditions for cardiac repair in the ovine model.
Indexed as
Identifiers
23539675PMC3607208W2114674527What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.