ArticleSpringerPlus2013
Comparison of a quantitative real-time polymerase chain reaction (qPCR) with conventional PCR, bacterial culture and ELISA for detection of Mycobacterium avium subsp. paratuberculosis infection in sheep showing pathology of Johne's disease.
Article in SpringerPlus, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 34 citations in OpenAlex.
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- Influence of untreated and treated recycled coarse aggregates on engineering properties of slag dolomite geopolymer concrete.Scientific reports · 2025Article
- High throughput screening for human disease associated-pathogens and antimicrobial resistance genes in migratory birds at ten habitat sites in China.BMC microbiology · 2025Article
- Histo-Pathological and Serological Investigation on the Occurrence of Subclinical Paratuberculosis in Slaughtered Buffaloes in Mhow, Madhya Pradesh, India.Indian journal of microbiology · 2025Article
- One-Step Multiplex Real-Time Fluorescent Quantitative Reverse Transcription PCR for Simultaneous Detection of Four Waterfowl Viruses.Microorganisms · 2024Article
- Improved DNA Amplification of the Hallmark ISApplied and environmental microbiology · 2023Article
- Serological and Molecular Characterization ofAnimals : an open access journal from MDPI · 2021Article
- Compared with conventional PCR assay, qPCR assay greatly improves the detection efficiency of predation.Ecology and evolution · 2020Article
- An update on Mycobacterium avium subspecies paratuberculosis antigens and their role in the diagnosis of Johne's disease.World journal of microbiology & biotechnology · 2019Review
- Recruitment of cyanobacteria by reverse transcription quantitative real-time PCR based on expression ofPeerJ · 2019Article
- Validation of an in-house real-time PCR fecal assay and comparison with two commercial assays for the antemortem detection of Mycobacterium avium subsp. paratuberculosis infection in culled sheep.Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2019Article
- Current status ofThe Indian journal of medical research · 2016Review
- Detection of Mycobacterium avium subsp. paratuberculosis in bovine milk from the state of Pernambuco, Brazil.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]Article
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2 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
A quantitative real-time PCR (qPCR) assay employing IS900 gene specific primers of Mycobacterium avium subsp. parartuberculosis (MAP) was compared with conventional PCR, bacterial culture and enzyme-linked immunosorbent assay in 38 sheep showing granulomatous enteritis and lymphadenitis with and without demonstration of acid-fast bacilli (AFB). The lesions were classified as multibacillary (MB) (n = 23), which had diffuse granulomatous lesions with abundant AFB, and paucibacillary (PB) (n = 15), which had focal or multifocal granulomatous lesions with few or no AFB. In the multibacillary group (MB), IS900 PCR detected 19 (82.6%), and qPCR detected all 23 (100%) sheep positive for MAP in the intestine and lymph node tissues. In the paucibacillary group (PB), IS900 PCR detected 2 (13.3%), and qPCR detected all 15 (100%) sheep positive for MAP in tissues. When results of both groups were taken together, IS900 PCR detected 21(55.2%), and qPCR detected all 38 (100%) animals positive for MAP genome either in the intestine or lymph node tissues. On Herrold egg yolk medium, tissues of 14 (60.9%) MB and 5 (33.3%) PB sheep were found to be positive for MAP. Out of 27 sheep (PB = 8, MB = 19) tested by an ELISA, 21 (77.7%) were found to be positive for MAP antibody, of which 25% (2/8) and 100% (19/19) sheep were from PB and MB sheep, respectively. Based on the results of the present study, it was concluded that qPCR was a highly sensitive test in comparison to conventional PCR, ELISA and bacterial culture for the diagnosis of paratuberculosis on infected tissues especially from paucibacillary sheep.
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