ArticleNature protocols2013
Isolation and in vitro culture of primary cardiomyocytes from adult zebrafish hearts.
Article in Nature protocols, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
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Who cites it
51 citing papers in PubMed, 79 citations in OpenAlex.
- WASHC3 knockout disrupts mitochondrial protein homeostasis and energy metabolism in cardiomyocytes.Frontiers in cardiovascular medicine · 2026Article
- Reactivation of an embryonic cardiac neural crest transcriptional profile during zebrafish heart regeneration.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Beating Cardiac Cell Cultures From Different Developmental Stages of Rainbow Trout as a Novel Approach for Replication of Cardiac Fish Viruses.Journal of fish diseases · 2025Article
- An organ-wide spatiotemporal transcriptomic and cellular atlas of the regenerating zebrafish heart.Nature communications · 2025Article
- Bioprinting approaches in cardiac tissue engineering to reproduce blood-pumping heart function.iScience · 2025Review
- The transcriptional repressor HEY2 regulates mitochondrial oxidative respiration to maintain cardiac homeostasis.Nature communications · 2025Article
- Use of cardiac cell cultures from salmonids to measure the cardiotoxic effect of environmental pollutants.Journal of fish diseases · 2025Article
- Zebrafish as a Model System for Brugada Syndrome.Reviews in cardiovascular medicine · 2024Review
- Transient titin-dependent ventricular defects during development lead to adult atrial arrhythmia and impaired contractility.iScience · 2024Article
- Deficiency of heme oxygenase 1a causes detrimental effects on cardiac function.Journal of cellular and molecular medicine · 2024Article
- Zebrafish as a Model for Cardiovascular and Metabolic Disease: The Future of Precision Medicine.Biomedicines · 2024Review
- Single-cell chromatin profiling reveals genetic programs activating proregenerative states in nonmyocyte cells.Science advances · 2024Article
- The Role ofCirculation research · 2024Article
- The ion channel Trpc6a regulates the cardiomyocyte regenerative response to mechanical stretch.Frontiers in cardiovascular medicine · 2023Article
- Consecutive treatments of methamphetamine promote the development of cardiac pathological symptoms in zebrafish.PloS one · 2023Article
- Ultrasound-targeted microbubble destruction (UTMD)-mediated miR-150-5p attenuates oxygen and glucose deprivation-induced cardiomyocyte injury by inhibiting TTC5 expression.Molecular biology reports · 2022Article
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- Isolation of Cardiac and Vascular Smooth Muscle Cells from Adult, Juvenile, Larval and Embryonic Zebrafish for Electrophysiological Studies.Journal of visualized experiments : JoVE · 2022Article
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Corrections and comments
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Authors and funding
5 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This protocol describes how to isolate primary cardiomyocytes from adult zebrafish hearts and culture them for up to 4 weeks, thereby using them as an alternative to in vivo experiments. After collagenase digestion of the ventricle, cells are exposed to increasing calcium concentrations in order to obtain high-purity cardiomyocytes. The whole isolation process can be accomplished in 4-5 h. The culture conditions we established allow the cells to preserve their mature sarcomeric integrity and contractile properties. Furthermore, adult zebrafish cardiomyocytes in culture, similarly to zebrafish in vivo heart regeneration, undergo partial dedifferentiation and, in contrast to their mammalian counterparts, are able to proliferate. Our protocol enables the study of structural and functional properties in close-to-native cardiomyocytes and allows the application of in vitro techniques and assays that are not feasible to perform in living animals.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.