ArticleNature protocols2013
Qualitative and quantitative metabolomic investigation of single neurons by capillary electrophoresis electrospray ionization mass spectrometry.
Article in Nature protocols, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
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Who cites it
58 citing papers in PubMed.
- Electrospray Regime Controls Sensitivity and Quantitative Performance in Micro- and Nano-Flow CE-ESI-MS.Analytical chemistry · 2026Article
- Single-bacterium metabolome revealing heterogeneous cellular states in bacterial populations.Nature communications · 2026Article
- Multimodal mass spectrometry imaging for plaque- and region-specific neurolipidomics in Alzheimer's disease mouse models.Nature communications · 2025Article
- Real-Time Eco-AI, Electrophoresis-Correlative Data-Dependent Acquisition with AI-Based Data Processing Broadens Access to Single-Cell Mass Spectrometry Proteomics.Angewandte Chemie (International ed. in English) · 2025Article
- Capillary Electrophoresis Electrospray Ionization Mass Spectrometry Reveals Metabolic Perturbations During Nematode Infection inMolecules (Basel, Switzerland) · 2025Article
- Combining transcriptomic and metabolomic insights to guide the clinical application of adipose- and bone marrow-derived mesenchymal stem cells.Medical review (2021) · 2025Article
- Neurochemical and Morphological Comparisons of Motor Nerve Organoids and Spinal-Cord Explants.ACS chemical neuroscience · 2025Article
- Unravelling lipid heterogeneity: Advances in single-cell lipidomics in cellular metabolism and disease.BBA advances · 2025Review
- Approaches to investigate tissue-resident innate lymphocytes metabolism at the single-cell level.Nature communications · 2024Review
- Opportunities and Challenges in Advancing Plant Research with Single-cell Omics.Genomics, proteomics & bioinformatics · 2024Review
- Biomolecular Actions by Intestinal Endotoxemia in Metabolic Syndrome.International journal of molecular sciences · 2024Review
- Electrochemically Etched Tapered-Tip Stainless-Steel Electrospray-Ionization Emitters for Capillary Electrophoresis-Mass Spectrometry.Journal of proteome research · 2023Article
- Zero-Degree Celsius Capillary Electrophoresis Electrospray Ionization for Hydrogen Exchange Mass Spectrometry.Analytical chemistry · 2023Article
- Enantioselective CE-MS analysis of ketamine metabolites in urine.Electrophoresis · 2023Article
- Cell-Lineage Guided Mass Spectrometry Proteomics in the Developing (Frog) Embryo.Journal of visualized experiments : JoVE · 2022Article
- Single-Cell Mass Spectrometry of Metabolites and Proteins for Systems and Functional Biology.Neuromethods · 2022Article
- In Vivo Subcellular Mass Spectrometry Enables Proteo-Metabolomic Single-Cell Systems Biology in a Chordate Embryo Developing to a Normally Behaving Tadpole (X. laevis)*.Angewandte Chemie (International ed. in English) · 2021Article
- Analysis of Peptide Stereochemistry in Single Cells by Capillary Electrophoresis-Trapped Ion Mobility Spectrometry Mass Spectrometry.Analytical chemistry · 2021Article
- Spray-Capillary-Based Capillary Electrophoresis Mass Spectrometry for Metabolite Analysis in Single Cells.Analytical chemistry · 2021Article
- Lipidomic profiling of single mammalian cells by infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI).Analytical and bioanalytical chemistry · 2020Article
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Authors and funding
4 authors.
Funding
Abstract
Single-cell mass spectrometry (MS) empowers metabolomic investigations by decreasing analytical dimensions to the size of individual cells and subcellular structures. We describe a protocol for investigating and quantifying metabolites in individual isolated neurons using single-cell capillary electrophoresis (CE) coupled to electrospray ionization (ESI) time-of-flight (TOF) MS. The protocol requires ∼2 h for sample preparation, neuron isolation and metabolite extraction, and 1 h for metabolic measurement. We used the approach to detect more than 300 distinct compounds in the mass range of typical metabolites in various individual neurons (25-500 μm in diameter) isolated from the sea slug (Aplysia californica) central and rat (Rattus norvegicus) peripheral nervous systems. We found that a subset of identified compounds was sufficient to reveal metabolic differences among freshly isolated neurons of different types and changes in the metabolite profiles of cultured neurons. The protocol can be applied to the characterization of the metabolome in a variety of smaller cells and/or subcellular domains.
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