ArticleFASEB journal : official publication of the Federation of American Societies for Experimental Biology2013
Simultaneously defining cell phenotypes, cell cycle, and chromatin modifications at single-cell resolution.
Article in FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed, 35 citations in OpenAlex.
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- Integrated -omics approach reveals persistent DNA damage rewires lipid metabolism and histone hyperacetylation via MYS-1/Tip60.Science advances · 2022Article
- A robust unsupervised machine-learning method to quantify the morphological heterogeneity of cells and nuclei.Nature protocols · 2021Article
- Transforming early pharmaceutical assessment of genotoxicity: applying statistical learning to a high throughput, multi end point in vitro micronucleus assay.Scientific reports · 2021Article
- The Relative Densities of Cytoplasm and Nuclear Compartments Are Robust against Strong Perturbation.Biophysical journal · 2020Article
- Biological Aging Modulates Cell Migration via Lamin A/C-Dependent Nuclear Motion.Micromachines · 2020Article
- Single-cell morphology encodes metastatic potential.Science advances · 2020Article
- Matrix Mechanics Influence Fibroblast-Myofibroblast Transition by Directing the Localization of Histone Deacetylase 4.Cellular and molecular bioengineering · 2017Article
- Biophysical and biomolecular determination of cellular age in humans.Nature biomedical engineering · 2017Article
- Morphological features of IFN-γ-stimulated mesenchymal stromal cells predict overall immunosuppressive capacity.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- MMSET/WHSC1 enhances DNA damage repair leading to an increase in resistance to chemotherapeutic agents.Oncogene · 2016Article
- A high-content image-based method for quantitatively studying context-dependent cell population dynamics.Scientific reports · 2016Article
- PAC1R agonist maxadilan enhances hADSC viability and neural differentiation potential.Journal of cellular and molecular medicine · 2016Article
- Biophysical changes reduce energetic demand in growth factor-deprived lymphocytes.The Journal of cell biology · 2016Article
- Evolution of cellular morpho-phenotypes in cancer metastasis.Scientific reports · 2015Article
- Normal mammary epithelial cells promote carcinoma basement membrane invasion by inducing microtubule-rich protrusions.Oncotarget · 2015Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Heterogeneity of cellular phenotypes in asynchronous cell populations placed in the same biochemical and biophysical environment may depend on cell cycle and chromatin modifications; however, no current method can measure these properties at single-cell resolution simultaneously and in situ. Here, we develop, test, and validate a new microscopy assay that rapidly quantifies global acetylation on histone H3 and measures a wide range of cell and nuclear properties, including cell and nuclear morphology descriptors, cell-cycle phase, and F-actin content of thousands of cells simultaneously, without cell detachment from their substrate, at single-cell resolution. These measurements show that isogenic, isotypic cells of identical DNA content and the same cell-cycle phase can still display large variations in H3 acetylation and that these variations predict specific phenotypic variations, in particular, nuclear size and actin cytoskeleton content, but not cell shape. The dependence of cell and nuclear properties on cell-cycle phase is assessed without artifact-prone cell synchronization. To further demonstrate its versatility, this assay is used to quantify the complex interplay among cell cycle, epigenetic modifications, and phenotypic variations following pharmacological treatments affecting DNA integrity, cell cycle, and inhibiting chromatin-modifying enzymes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.