ArticlePloS one2013
Protein kinase G1 α overexpression increases stem cell survival and cardiac function after myocardial infarction.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 26 citations in OpenAlex.
- Regulation and Pharmacology of the Cyclic GMP and Nitric Oxide Pathway in Embryonic and Adult Stem Cells.Cells · 2024Review
- Carvedilol Activates a Myofilament Signaling Circuitry to Restore Cardiac Contractility in Heart Failure.JACC. Basic to translational science · 2024Article
- Article
- Substance P enhances the therapeutic effect of MSCs by modulating their angiogenic potential.Journal of cellular and molecular medicine · 2020Article
- Pharmacological preconditioning with phosphodiestrase inhibitor: an answer to stem cell survival against ischemic injury through JAK/STAT signaling.Heart failure reviews · 2020Review
- SDF1 gradient associates with the distribution of c-Kit+ cardiac cells in the heart.Scientific reports · 2018Article
- Functionally Improved Mesenchymal Stem Cells to Better Treat Myocardial Infarction.Stem cells international · 2018Review
- Integrin β1 Increases Stem Cell Survival and Cardiac Function after Myocardial Infarction.Frontiers in pharmacology · 2017Article
- Protective effect of TSG against oxygen-glucose deprivation in cardiomyoblast cell line H9c2: involvement of Bcl-2 family, Caspase 3/9, and Akt signaling pathway.International journal of clinical and experimental pathology · 2017Article
- Combined MSC and GLP-1 Therapy Modulates Collagen Remodeling and Apoptosis following Myocardial Infarction.Stem cells international · 2016Article
- Genetic Engineering of Mesenchymal Stem Cells to Induce Their Migration and Survival.Stem cells international · 2016Review
- Tadalafil, a Phosphodiesterase Inhibitor Protects Stem Cells over Longer Period Against Hypoxia/Reoxygenation Injury Through STAT3/PKG-I Signaling.Stem cells and development · 2015Article
- "Second-generation" stem cells for cardiac repair.World journal of stem cells · 2015Review
- Human mesenchymal stem cells/multipotent stromal cells consume accumulated autophagosomes early in differentiation.Stem cell research & therapy · 2014Article
- Preconditioning of bone marrow mesenchymal stem cells by prolyl hydroxylase inhibition enhances cell survival and angiogenesis in vitro and after transplantation into the ischemic heart of rats.Stem cell research & therapy · 2014Article
- Cardiac stem cell therapy for cardiac repair.Current treatment options in cardiovascular medicine · 2014Article
- Impaired autophagy contributes to adverse cardiac remodeling in acute myocardial infarction.PloS one · 2014Article
- Activity of mesenchymal stem cells in therapies for chronic skin wound healing.Organogenesis · 2014Review
- Mesenchymal Stem Cell Paracrine Factors in Vascular Repair and Regeneration.Journal of biomedical technology and research · 2014Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
backgroundWe hypothesized that overexpression of cGMP-dependent protein kinase type 1α (PKG1α) could mimic the effect of tadalafil on the survival of bone marrow derived mesenchymal stem cells (MSCs) contributing to regeneration of the ischemic heart. METHODS AND
resultsMSCs from male rats were transduced with adenoviral vector encoding for PKG1α ((PKG1α)MSCs).Controls included native MSCs ((Nat)MSCs) and MSCs transduced with an empty vector ((Null)MSCs). PKG1α activity was increased approximately 20, 5 and 16 fold respectively in (PKG1α)MSCs. (PKG1α)MSCs showed improved survival under oxygen and glucose deprivation (OGD) which was evidenced by lower LDH release, caspase-3/7 activity and number of positive TUNEL cells. Anti-apoptotic proteins pAkt, pGSK3β, and Bcl-2 were significantly increased in (PKG1α)MSCs compared to (Nat)MSCs and (Null)MSCs. Higher release of multiple prosurvival and angiogenic factors such as HGF, bFGF, SDF-1 and Ang-1 was observed in (PKG1α)MSCs before and after OGD. In a female rat model of acute myocardial infarction, (PKG1α)MSCs group showed higher survival compared with (Null)MSCs group at 3 and 7 days after transplantation as determined by TUNEL staining and sry-gene quantitation by real-time PCR. Increased anti-apoptotic proteins and paracrine factors in vitro were also identified. Immunostaining for cardiac troponin I combined with GFP showed increased myogenic differentiation of (PKG1α)MSCs. At 4 weeks after transplantation, compared to DMEM group and (Null)MSCs group, (PKG1α)MSCs group showed increased blood vessel density in infarct and peri-infarct areas (62.5±7.7; 68.8±7.3 per microscopic view, p<0.05) and attenuated infarct size (27.2±2.5%, p<0.01). Heart function indices including ejection fraction (52.1±2.2%, p<0.01) and fractional shortening (24.8%±1.3%, p<0.01) were improved significantly in (PKG1α)MSCs group.
conclusionOverexpression of PKG1α transgene could be a powerful approach to improve MSCs survival and their angiomyogenic potential in the infarcted heart.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.