ArticlePloS one2013
Identification and Characterisation CRN Effectors in Phytophthora capsici Shows Modularity and Functional Diversity.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 90 papers.
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Who cites it
90 citing papers in PubMed, 176 citations in OpenAlex.
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- AACRNL evolved from virulence factor to epigenetic parasite driving genome expansion in free-living eukaryotes.Nature communications · 2026Article
- Intraspecies sequence-graph analysis of the Phytophthora theobromicola genome reveals a dynamic structure and variable effector repertoires.G3 (Bethesda, Md.) · 2026Article
- Comprehensive whole genome hybrid assembly unravels genes and secretome ofFrontiers in fungal biology · 2026Article
- Profiling the Genomes and Secreted Effector Proteins inJournal of fungi (Basel, Switzerland) · 2025Article
- Decoding Plant-Pathogen Interactions: A Comprehensive Exploration of Effector-Plant Transcription Factor Dynamics.Molecular plant pathology · 2025Review
- Effector-Mediated Suppression of Programmed Cell Death byJournal of fungi (Basel, Switzerland) · 2024Article
- Dual transcriptional characterization of spinach and Peronospora effusa during resistant and susceptible race-cultivar interactions.BMC genomics · 2024Article
- Identification of Phytophthora cinnamomi CRN effectors and their roles in manipulating cell death during Persea americana infection.BMC genomics · 2024Article
- Characterization of Arbuscular Mycorrhizal Effector Proteins.International journal of molecular sciences · 2023Review
- High-throughput time series expression profiling of Plasmopara halstedii infecting Helianthus annuus reveals conserved sequence motifs upstream of co-expressed genes.BMC genomics · 2023Article
- Fungal effectors versus defense-related genes ofFrontiers in plant science · 2023Review
- Progress and Challenges in Elucidating the Functional Role of Effectors in the Soybean-Journal of fungi (Basel, Switzerland) · 2022Review
- Virulence strategies of an insect herbivore and oomycete plant pathogen converge on host E3 SUMO ligase SIZ1.The New phytologist · 2022Article
- Unraveling Plant Cell Death duringMicroorganisms · 2022Review
- Strive or thrive: Trends inFrontiers in plant science · 2022Article
- Comparative Genome Analysis Across 128Frontiers in microbiology · 2022Article
- Chromosome-level assembly of theFrontiers in microbiology · 2022Article
- Differing Responses toFrontiers in plant science · 2022Article
30 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Phytophthora species secrete a large array of effectors during infection of their host plants. The Crinkler (CRN) gene family encodes a ubiquitous but understudied class of effectors with possible but as of yet unknown roles in infection. To appreciate CRN effector function in Phytophthora, we devised a simple Crn gene identification and annotation pipeline to improve effector prediction rates. We predicted 84 full-length CRN coding genes and assessed CRN effector domain diversity in sequenced Oomycete genomes. These analyses revealed evidence of CRN domain innovation in Phytophthora and expansion in the Peronosporales. We performed gene expression analyses to validate and define two classes of CRN effectors, each possibly contributing to infection at different stages. CRN localisation studies revealed that P. capsici CRN effector domains target the nucleus and accumulate in specific sub-nuclear compartments. Phenotypic analyses showed that few CRN domains induce necrosis when expressed in planta and that one cell death inducing effector, enhances P. capsici virulence on Nicotiana benthamiana. These results suggest that the CRN protein family form an important class of intracellular effectors that target the host nucleus during infection. These results combined with domain expansion in hemi-biotrophic and necrotrophic pathogens, suggests specific contributions to pathogen lifestyles. This work will bolster CRN identification efforts in other sequenced oomycete species and set the stage for future functional studies towards understanding CRN effector functions.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.