ArticlePloS one2013
NR5A2 regulates Lhb and Fshb transcription in gonadotrope-like cells in vitro, but is dispensable for gonadotropin synthesis and fertility in vivo.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 24 citations in OpenAlex.
- Co-profiling of single-cell gene expression and chromatin landscapes in stickleback pituitary.Scientific data · 2025Article
- Clinical and Molecular Characteristics of Gonadotroph Pituitary Tumors According to the WHO Classification.Endocrine pathology · 2024Article
- Pituitary Gonadotropin Gene Expression During Induced Onset of Postsmolt Maturation in Male Atlantic Salmon:Frontiers in endocrinology · 2022Article
- The Hippo Pathway Effectors YAP and TAZ Regulate LH Release by Pituitary Gonadotrope Cells in Mice.Endocrinology · 2022Article
- Nuclear Receptors as Regulators of Pituitary Corticotroph Pro-Opiomelanocortin Transcription.Cells · 2020Review
- Gonadotrope-specific deletion of the BMP type 2 receptor does not affect reproductive physiology in mice†‡.Biology of reproduction · 2020Article
- Cell Type- and Sex-Dependent Transcriptome Profiles of Rat Anterior Pituitary Cells.Frontiers in endocrinology · 2019Article
- Sex- and Age-Specific Impact of ERK Loss Within the Pituitary Gonadotrope in Mice.Endocrinology · 2018Article
- Regulatory Architecture of the LβT2 Gonadotrope Cell Underlying the Response to Gonadotropin-Releasing Hormone.Frontiers in endocrinology · 2018Article
- Characterization of Gonadotrope Secretoproteome Identifies Neurosecretory Protein VGF-derived Peptide Suppression of Follicle-stimulating Hormone Gene Expression.The Journal of biological chemistry · 2016Article
- Normal gonadotropin production and fertility in gonadotrope-specific Bmpr1a knockout mice.The Journal of endocrinology · 2016Article
- Minireview: Activin Signaling in Gonadotropes: What Does the FOX say… to the SMAD?Molecular endocrinology (Baltimore, Md.) · 2015Review
- β-catenin stabilization in gonadotropes impairs FSH synthesis in male mice in vivo.Endocrinology · 2015Article
- Follicle-stimulating hormone synthesis and fertility depend on SMAD4 and FOXL2.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2014Article
- Follicle-stimulating hormone synthesis and fertility are intact in mice lacking SMAD3 DNA binding activity and SMAD2 in gonadotrope cells.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2014Article
- The CpG island in the murine foxl2 proximal promoter is differentially methylated in primary and immortalized cells.PloS one · 2013Article
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Authors and funding
9 authors at 4 institutions in 3 countries.
Funding
Abstract
Successful mammalian reproduction depends on proper synthesis of the pituitary-derived glycoprotein hormones, luteinizing hormone (LH) and follicle-stimulating hormone (FSH). Several transcription factors cooperate to activate cell-specific and hormone-regulated expression of the gonadotropin beta subunits (Lhb and Fshb). Among these, NR5A1 (steroidogenic factor 1; SF1) has been shown to directly bind to the Lhb promoter, mediate basal and gonadotropin-releasing hormone (GnRH)-stimulated Lhb transcription, and possibly directly regulate Fshb expression. Recently, the closely-related NR5A2 was shown to activate the rat Lhb promoter in vitro. Here, we further characterized the role of NR5A2 in regulating gonadotropin synthesis. Ectopically expressed NR5A2 directly activated the murine Lhb promoter in a manner identical to that of NR5A1, whereas neither factor activated the murine Fshb promoter. In LβT2 gonadotrope-like cells, depletion of endogenous NR5A1 or NR5A2 impaired basal and GnRH-stimulated Lhb and Fshb transcription. To analyze the physiological role of NR5A2 in gonadotropes in vivo, we generated mice with a gonadotrope-specific deletion of Nr5a2. In contrast with our in vitro data, these mice had normal pituitary Lhb and Fshb expression and intact fertility. Together, our data establish that NR5A2 can act in a non-redundant manner to regulate Lhb and Fshb transcription in vitro, but is dispensable in vivo.
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