ArticlePloS one2013
Proteomic biomarkers for ageing the mosquito Aedes aegypti to determine risk of pathogen transmission.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 40 citations in OpenAlex.
- Towards scalable age-grading of Aedes albopictus mosquito using mid-infrared spectroscopy and machine learning.Scientific reports · 2025Article
- Pixel intensity of wing photos used to infer efficacy of mosquito control interventions against Anopheles gambiae caught during the RIMDAMAL II clinical trial.Scientific reports · 2025Article
- Hologenomic structure of bacterial and fungal community composition in the West Nile virus vectorbioRxiv : the preprint server for biology · 2025Article
- Predicting the age of fieldScience advances · 2024Article
- Article
- Utility of MALDI-TOF MS for determination of species identity and blood meal sources of primary malaria vectors on the Kenyan coast.Wellcome open research · 2023Article
- Life as a Vector of Dengue Virus: The Antioxidant Strategy of Mosquito Cells to Survive Viral Infection.Antioxidants (Basel, Switzerland) · 2021Review
- Time Flies-Age Grading of Adult Flies for the Estimation of the Post-Mortem Interval.Diagnostics (Basel, Switzerland) · 2021Review
- Article
- First report on the application of near-infrared spectroscopy to predict the age of Aedes albopictus Skuse.Scientific reports · 2018Article
- Fat Body Organ Culture System in Aedes Aegypti, a Vector of Zika Virus.Journal of visualized experiments : JoVE · 2017Article
- Complexity of virus-vector interactions.Current opinion in virology · 2016Review
- Metabolomics of aging assessed in individual parasitoid wasps.Scientific reports · 2016Article
- Near-Infrared Spectroscopy, a Rapid Method for Predicting the Age of Male and Female Wild-Type and Wolbachia Infected Aedes aegypti.PLoS neglected tropical diseases · 2016Article
- Mass spectrometry identification of age-associated proteins from the malaria mosquitoes Anopheles gambiae s.s. and Anopheles stephensi.Data in brief · 2015Article
- Rapid protein profiling facilitates surveillance of invasive mosquito species.Parasites & vectors · 2014Article
Corrections and comments
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Authors and funding
11 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Biomarkers of the age of mosquitoes are required to determine the risk of transmission of various pathogens as each pathogen undergoes a period of extrinsic incubation in the mosquito host. Using the 2-D Difference Gel Electrophoresis (2-D DIGE) procedure, we investigated the abundance of up to 898 proteins from the Yellow Fever and dengue virus vector, Aedes aegypti, during ageing. By applying a mixed-effects model of protein expression, we identified five common patterns of abundance change during ageing and demonstrated an age-related decrease in variance for four of these. This supported a search for specific proteins with abundance changes that remain tightly associated with ageing for use as ageing biomarkers. Using MALDI-TOF/TOF mass spectrometry we identified ten candidate proteins that satisfied strict biomarker discovery criteria (identified in two out of three multivariate analysis procedures and in two cohorts of mosquitoes). We validated the abundances of the four most suitable candidates (Actin depolymerising factor; ADF, Eukaryotic initiation factor 5A; eIF5A, insect cuticle protein Q17LN8, and Anterior fat body protein; AFP) using semi-quantitative Western analysis of individual mosquitoes of six ages. The redox-response protein Manganese superoxide dismutase (SOD2) and electron shuttling protein Electron transfer oxidoreductase (ETO) were subject to post-translational modifications affecting their charge states with potential effects on function. For the four candidates we show remarkably consistent decreases in abundance during ageing, validating initial selections. In particular, the abundance of AFP is an ideal biomarker candidate for whether a female mosquito has lived long enough to be capable of dengue virus transmission. We have demonstrated proteins to be a suitable class of ageing biomarkers in mosquitoes and have identified candidates for epidemiological studies of dengue and the evaluation of new disease reduction projects targeting mosquito longevity.
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