ArticlePloS one2013
Amino acid substitutions in cold-adapted proteins from Halorubrum lacusprofundi, an extremely halophilic microbe from antarctica.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 37 papers.
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37 citing papers in PubMed, 74 citations in OpenAlex.
- Giant viruses of the polar regions: diversity, endemism, adaptation and ecological structuring.FEMS microbiology ecology · 2026Review
- Coordinated proteome-scale remodeling underlies polyextremophilic survival in Antarctic cryo-hypersaline brines.Frontiers in microbiology · 2026Article
- Structural and Functional Variations of Enzymes Indicate Latitudinal Adaptations of Subarctic and Arctic Sea Urchins.Ecology and evolution · 2025Article
- Molecular Fingerprint of Cold Adaptation in Antarctic Icefish PepT1 (Biomolecules · 2025Article
- Molecular Evolution and Adaptation Strategies in Marine Ciliates: An Inspiration for Cold-Adapted Enzyme Engineering and Drug Binding Analysis.Marine drugs · 2024Review
- Adaptation strategies of giant viruses to low-temperature marine ecosystems.The ISME journal · 2024Article
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- Improving the genetic system forFrontiers in microbiology · 2023Article
- Effects of Salinity and Temperature on the Flexibility and Function of a Polyextremophilic Enzyme.International journal of molecular sciences · 2022Article
- Coevolution of the Ess1-CTD axis in polar fungi suggests a role for phase separation in cold tolerance.Science advances · 2022Article
- Extracellular Enzymatic Activities of Oceanic Pelagic Fungal Strains and the Influence of Temperature.Journal of fungi (Basel, Switzerland) · 2022Article
- Double mutations far from the active site affect cold activity in an Antarctic halophilic β-galactosidase.Protein science : a publication of the Protein Society · 2022Article
- Comparative Genomic Analyses of the GenusMicroorganisms · 2022Article
- Hypersaline environments as natural sources of microbes with potential applications in biotechnology: The case of solar evaporation systems to produce salt in Alicante County (Spain).Current research in microbial sciences · 2022Review
- Extremophile enzyme optimization for low temperature and high salinity are fundamentally incompatible.Extremophiles : life under extreme conditions · 2021Article
- Cold Adaptation Strategies and the Potential of Psychrophilic Enzymes from the Antarctic Yeast,Journal of fungi (Basel, Switzerland) · 2021Review
- Bioprospecting of Novel Extremozymes From Prokaryotes-The Advent of Culture-Independent Methods.Frontiers in microbiology · 2021Review
- Understanding High-Salt and Cold Adaptation of a Polyextremophilic Enzyme.Microorganisms · 2020Article
- Extremophilic models for astrobiology: haloarchaeal survival strategies and pigments for remote sensing.Extremophiles : life under extreme conditions · 2020Review
Corrections and comments
- Erratum issued
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The halophilic Archaeon Halorubrum lacusprofundi, isolated from the perennially cold and hypersaline Deep Lake in Antarctica, was recently sequenced and compared to 12 Haloarchaea from temperate climates by comparative genomics. Amino acid substitutions for 604 H. lacusprofundi proteins belonging to conserved haloarchaeal orthologous groups (cHOGs) were determined and found to occur at 7.85% of positions invariant in proteins from mesophilic Haloarchaea. The following substitutions were observed most frequently: (a) glutamic acid with aspartic acid or alanine; (b) small polar residues with other small polar or non-polar amino acids; (c) small non-polar residues with other small non-polar residues; (d) aromatic residues, especially tryptophan, with other aromatic residues; and (e) some larger polar residues with other similar residues. Amino acid substitutions for a cold-active H. lacusprofundi β-galactosidase were then examined in the context of a homology modeled structure at residues invariant in homologous enzymes from mesophilic Haloarchaea. Similar substitutions were observed as in the genome-wide approach, with the surface accessible regions of β-galactosidase displaying reduced acidity and increased hydrophobicity, and internal regions displaying mainly subtle changes among smaller non-polar and polar residues. These findings are consistent with H. lacusprofundi proteins displaying amino acid substitutions that increase structural flexibility and protein function at low temperature. We discuss the likely mechanisms of protein adaptation to a cold, hypersaline environment on Earth, with possible relevance to life elsewhere.
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