ArticleMolecular biology of the cell2013
Divergent modes for cargo-mediated control of clathrin-coated pit dynamics.
Article in Molecular biology of the cell, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
26 citing papers in PubMed, 30 citations in OpenAlex.
- Local GPCR density tips the balance of μ-opioid receptor trafficking.bioRxiv : the preprint server for biology · 2026Article
- Effects of two structurally diverse positive allosteric modulators on signaling bias at the μ-opioid receptor.Molecular pharmacology · 2025Article
- Membrane order regulates clathrin-coated pit dynamics but not initiation.Molecular biology of the cell · 2025Article
- Vesicle-associated membrane protein 2 is a cargo-selective v-SNARE for a subset of GPCRs.The Journal of cell biology · 2023Article
- Endocytosis in the context-dependent regulation of individual and collective cell properties.Nature reviews. Molecular cell biology · 2021Review
- A New Paroxetine-Based GRK2 Inhibitor Reduces Internalization of theMolecular pharmacology · 2020Article
- Homologous Regulation of Mu Opioid Receptor Recycling by GMolecular pharmacology · 2019Article
- Spatial encoding of GPCR signaling in the nervous system.Current opinion in cell biology · 2019Review
- Regulation of G protein-coupled receptor signaling by plasma membrane organization and endocytosis.Traffic (Copenhagen, Denmark) · 2019Review
- Cargo regulates clathrin-coated pit invagination via clathrin light chain phosphorylation.The Journal of cell biology · 2018Article
- Membrane binding, internalization, and sorting of alpha-synuclein in the cell.Acta neuropathologica communications · 2018Article
- PI3K class II α regulates δ-opioid receptor export from theMolecular biology of the cell · 2017Article
- A PTEN-Regulated Checkpoint Controls Surface Delivery of δ Opioid Receptors.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2017Article
- Sequence-Specific Regulation of Endocytic Lifetimes Modulates Arrestin-Mediated Signaling at theMolecular pharmacology · 2017Article
- Distinct G protein-coupled receptor recycling pathways allow spatial control of downstream G protein signaling.The Journal of cell biology · 2016Article
- Endocytosis of Ubiquitylation-Deficient EGFR Mutants via Clathrin-Coated Pits is Mediated by Ubiquitylation.Traffic (Copenhagen, Denmark) · 2015Article
- Ubiquitin plays an atypical role in GPCR-induced p38 MAP kinase activation on endosomes.The Journal of cell biology · 2015Article
- Minireview: Spatial Programming of G Protein-Coupled Receptor Activity: Decoding Signaling in Health and Disease.Molecular endocrinology (Baltimore, Md.) · 2015Review
- Cell-autonomous regulation of Mu-opioid receptor recycling by substance P.Cell reports · 2015Article
- Real-time imaging of mu opioid receptors by total internal reflection fluorescence microscopy.Methods in molecular biology (Clifton, N.J.) · 2015Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Clathrin-mediated endocytosis has long been viewed as a process driven by core endocytic proteins, with internalized cargo proteins being passive. In contrast, an emerging view suggests that signaling receptor cargo may actively control its fate by regulating the dynamics of clathrin-coated pits (CCPs) that mediate their internalization. Despite its physiological implications, very little is known about such "cargo-mediated regulation" of CCPs by signaling receptors. Here, using multicolor total internal reflection fluorescence microscopy imaging and quantitative analysis in live cells, we show that the μ-opioid receptor, a physiologically relevant G protein-coupled signaling receptor, delays the dynamics of CCPs in which it is localized. This delay is mediated by the interactions of two critical leucines on the receptor cytoplasmic tail. Unlike the previously known mechanism of cargo-mediated regulation, these residues regulate the lifetimes of dynamin, a key component of CCP scission. These results identify a novel means for selectively controlling the endocytosis of distinct cargo that share common trafficking components and indicate that CCP regulation by signaling receptors can operate via divergent modes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.