ArticleReproductive sciences (Thousand Oaks, Calif.)2013
Embryonic stem cell-derived granulosa cells participate in ovarian follicle formation in vitro and in vivo.
Article in Reproductive sciences (Thousand Oaks, Calif.), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 31 citations in OpenAlex.
- New Solutions for Old Problems: How Reproductive Tissue Engineering Has Been Revolutionizing Reproductive Medicine.Annals of biomedical engineering · 2023Review
- Fertility restoration in mice with chemotherapy induced ovarian failure using differentiated iPSCs.EBioMedicine · 2023Article
- High-fidelity reprogramming into Leydig-like cells by CRISPR activation and paracrine factors.PNAS nexus · 2022Article
- Identification of Stem Cell-Like Cells in the Ovary.Tissue engineering and regenerative medicine · 2022Review
- Neurobehavioral alternations of the female offspring born to polycystic ovary syndrome model rats administered by Chinese herbal medicine.Chinese medicine · 2021Article
- Article
- In Vitro Generation of Oocytes from Ovarian Stem Cells (OSCs): In Search of Major Evidence.International journal of molecular sciences · 2019Review
- Review
- Female Fertility Preservation through Stem Cell-based Ovarian Tissue Reconstitution In Vitro and Ovarian Regeneration In Vivo.Clinical medicine insights. Reproductive health · 2019Review
- Exogenous leptin affects sperm parameters and impairs blood testis barrier integrity in adult male mice.Reproductive biology and endocrinology : RB&E · 2018Article
- An Embryonic and Induced Pluripotent Stem Cell Model for Ovarian Granulosa Cell Development and Steroidogenesis.Reproductive sciences (Thousand Oaks, Calif.) · 2018Article
- Ovarian regeneration: The potential for stem cell contribution in the postnatal ovary to sustained endocrine function.Molecular and cellular endocrinology · 2017Review
- Ovarian stem cells: From basic to clinical applications.World journal of stem cells · 2015Review
- Efficient differentiation of steroidogenic and germ-like cells from epigenetically-related iPSCs derived from ovarian granulosa cells.PloS one · 2015Article
- Ovarian-cell-like cells from skin stem cells restored estradiol production and estrus cycling in ovariectomized mice.Stem cells and development · 2014Article
- Comparative gene expression profiling of adult mouse ovary-derived oogonial stem cells supports a distinct cellular identity.Fertility and sterility · 2013Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Differentiating embryonic stem cells (ESCs) can form ovarian follicle-like structures in vitro, consisting of an oocyte-like cell surrounded by somatic cells capable of steroidogenesis. Using a dual-fluorescence reporter system in which mouse ESCs express green fluorescent protein (GFP) under the control of a germ cell-specific Pou5f1 gene promoter and red fluorescent protein (Discosoma sp red [DsRed]) driven by the granulosa cell-specific Forkhead box L2 (Foxl2) gene promoter, we first confirmed in vitro formation of follicle-like structures containing GFP-positive cells surrounded by DsRed-positive cells. Isolated DsRed-positive cells specified from ECSs exhibited a gene expression profile consistent with granulosa cells, as revealed by the detection of messenger RNAs (mRNAs) for Foxl2, follistatin (Fst), anti-Müllerian hormone (Amh), and follicle-stimulating hormone receptor (Fshr) as well as by production of both progesterone and estradiol. In addition, treatment of isolated DsRed-expressing cells with follicle-stimulating hormone (FSH) significantly increased estradiol production over basal levels, confirming the presence of functional FSH receptors in these cells. Last, ESC-derived DsRed-positive cells injected into neonatal mouse ovaries became incorporated within the granulosa cell layer of immature follicles. These studies demonstrate that Foxl2-expressing ovarian somatic cells derived in vitro from differentiating ESCs express granulosa cell markers, actively associate with germ cells in vitro, synthesize steroids, respond to FSH, and participate in folliculogenesis in vivo.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.