Evidence map›Paper›PMID 23536400›Full record

ArticleJournal of clinical microbiology2013

Development of a singleplex PCR assay for rapid identification and differentiation of Cryptococcus neoformans var. grubii, Cryptococcus neoformans var. neoformans, Cryptococcus gattii, and hybrids.

Xiaobo Feng, Xiaohua Fu, Bo Ling, Lei Wang, Wanqing Liao, Zhirong Yao

Abstract readEvaluation Study
In one paragraph

Article in Journal of clinical microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
  4. Article
  5. Phenotypic Diversity ofMicroorganisms · 2022
    Article
  6. The Canadian veterinary journal = La revue veterinaire canadienne · 2019
    Article
  7. Article
  8. Article
  9. Cryptococcus gattii infections.Clinical microbiology reviews · 2014
    Review
  10. Article
  11. Update on fungal diagnostics.Current infectious disease reports · 2014
    Article
  12. Article
  13. Article
  14. Skeletal cryptococcosis from 1977 to 2013.Frontiers in microbiology · 2014
    Review
  15. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Xiaobo FengMedical Mycology Laboratory, Department of Dermatology, Xinhua Hospital Affiliated to Shanghai Jiaotong University School of Medicine, Shanghai, China.
Xiaohua Fu
Bo Ling
Lei Wang
Wanqing Liao
Zhirong Yao

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A singleplex PCR assay using a single primer pair targeting the putative sugar transporter gene was developed here to distinguish Cryptococcus neoformans var. grubii, Cryptococcus neoformans var. neoformans, and Cryptococcus gattii according to the distinct size of the amplicon. The interspecies and intravarietal hybrids were also characterized on the basis of distinct combined profiles of amplicons. This PCR assay is a rapid, simple, and reliable approach suitable for laboratory diagnoses and large-scale epidemiologic studies.

Indexed as

Recombination, GeneticCryptococcosisCryptococcus gattiiCryptococcus neoformansDNA, FungalDNA PrimersHumansMicrobiological TechniquesMolecular Diagnostic TechniquesMolecular Sequence DataPolymerase Chain ReactionSequence Analysis, DNATime FactorsDNA, FungalDNA Primers

Identifiers

PMID23536400
PMCPMC3716086

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.