ArticleBiology of reproduction2013
Transgenerational effects of di-(2-ethylhexyl) phthalate on testicular germ cell associations and spermatogonial stem cells in mice.
Article in Biology of reproduction, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 102 papers, 2 of them syntheses that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
102 citing papers in PubMed, 2 syntheses or guidelines pooled it, 219 citations in OpenAlex.
- Systematic literature review and meta-analysis on the reproductive effects of micro- pollutants in humans and animals.Frontiers in toxicology · 2025Pooled it
- A systematic review on the adverse health effects of di-2-ethylhexyl phthalate.Environmental science and pollution research international · 2016Pooled it
- Prevalence and reproductive toxicity of di-2-ethylhexyl terephthalate (DEHTP) and other substituted benzene carboxylic acid derivatives used as phthalate alternatives.Environmental pollution (Barking, Essex : 1987) · 2026Review
- Di-(2-ethylhexyl) Phthalate Exposure Induces Developmental Toxicity in the Mouse Fetal Heart via Mitochondrial Dysfunction.Cardiovascular toxicology · 2025Article
- Generational stability of epigenetic transgenerational inheritance facilitates adaptation and evolution.Epigenetics · 2024Review
- Prenatal exposure to Di(2-ethylhexyl) phthalate and high-fat diet synergistically disrupts gonadal function in male mice†.Biology of reproduction · 2024Article
- Inter- and trans-generational impacts of real-world PMCell discovery · 2024Article
- Review
- Inheritance of environment-induced phenotypic changes through epigenetic mechanisms.Environmental epigenetics · 2023Review
- Identification of spermatogenesis-associated changes in DNA methylation induced by maternal exposure to chemicals in male germ cells.STAR protocols · 2022Article
- Screening of Potential Plasticizer Alternatives for Their Toxic Effects on Male Germline Stem Cells.Biomedicines · 2022Article
- Multi- and Transgenerational Effects of Environmental Toxicants on Mammalian Reproduction.Cells · 2022Review
- Prenatal and perinatal phthalate exposure is associated with sex-dependent changes in hippocampal miR-15b-5p and miR-34a-5p expression and changes in testicular morphology in rat offspring.Arhiv za higijenu rada i toksikologiju · 2022Article
- Article
- Multiple transcriptomic profiling: potential novel metabolism-related genes predict prepubertal testis damage caused by DEHP exposure.Environmental science and pollution research international · 2022Article
- Endocrine-Disrupting Chemicals and Their Adverse Effects on the Endoplasmic Reticulum.International journal of molecular sciences · 2022Review
- Developmental toxicant exposures and sex-specific effects on epigenetic programming and cardiovascular health across generations.Environmental epigenetics · 2022Review
- The Impact of Endocrine-Disrupting Chemicals in Male Fertility: Focus on the Action of Obesogens.Journal of xenobiotics · 2021Review
- Epi-mutations for spermatogenic defects by maternal exposure to di(2-ethylhexyl) phthalate.eLife · 2021Article
- First trimester mechanisms of gestational sac placental and foetal teratogenicity: a framework for birth cohort studies.Human reproduction update · 2021Review
42 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Recent evidence has linked human phthalate exposure to abnormal reproductive and hormonal effects. Phthalates are plasticizers that confer flexibility and transparency to plastics, but they readily contaminate the body and the environment. In this study, timed pregnant CD1 outbred mice were treated with di-(2-ethylhexyl) phthalate (DEHP) from Embryonic Day 7 (E7) to E14. The subsequent generation (F1) offspring were then bred to produce the F2, F3, and F4 offspring, without any further DEHP treatment. This exposure scheme disrupted testicular germ cell association and decreased sperm count and motility in F1 to F4 offspring. By spermatogonial transplantation techniques, the exposure scheme also disrupted spermatogonial stem cell (SSC) function of F3 offspring. The W/W(V) recipient testes transplanted with F3 offspring germ cells from the DEHP-treated group had a dramatically lower percentage of donor germ cell-derived spermatogenic recovery in seminiferous tubules when compared to the recipient testes transplanted with CD1 control germ cells. Further characterization showed that the major block of donor germ cell-derived spermatogenesis was before the appearance of undifferentiated spermatogonia. Interestingly, the testes transplanted with the F3 offspring germ cells from the DEHP-treated group, when regenerated, replicated testis morphology similar to that observed in the testes from the F1 to F3 offspring of the DEHP-treated group, suggesting that the germ cell disorganization phenotype originates from the stem cells of F3 offspring. In conclusion, embryonic exposure to DEHP was found to disrupt testicular germ cell organization and SSC function in a transgenerational manner.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.