ArticleProceedings of the National Academy of Sciences of the United States of America2013
Responses of Mn2+ speciation in Deinococcus radiodurans and Escherichia coli to γ-radiation by advanced paramagnetic resonance methods.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed.
- DrPitA-mediated enrichment of intracellular manganese and phosphate contributes to oxidative stress resistance ofApplied and environmental microbiology · 2026Article
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- Unravelling the developmental toxicity of heavy metals using zebrafish as a model: a narrative review.Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine · 2025Review
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- The ternary complex of MnProceedings of the National Academy of Sciences of the United States of America · 2024Article
- A water-soluble, cell-permeable Mn(ii) sensor enables visualization of manganese dynamics in live mammalian cells.Chemical science · 2024Article
- Terminal Hydride Complex of High-Spin Mn.Journal of the American Chemical Society · 2024Article
- Characterization of a Novel N4-Methylcytosine Restriction-Modification System inInternational journal of molecular sciences · 2024Article
- Structural and Functional Characterization of the Holliday Junction Resolvase RuvC fromMicroorganisms · 2022Article
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- Shining light on photosynthetic microbes and manganese-enriched rock varnish.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Metal ion fluxes controlling amphibian fertilization.Nature chemistry · 2021Article
- An ecophysiological explanation for manganese enrichment in rock varnish.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- A small RNA regulates pprM, a modulator of pleiotropic proteins promoting DNA repair, in Deinococcus radiodurans under ionizing radiation.Scientific reports · 2021Article
- Low-molecular-mass labile metal pools in Escherichia coli: advances using chromatography and mass spectrometry.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2021Article
- Intracellular Metal Speciation inACS infectious diseases · 2020Article
- The Manganese-Responsive Transcriptional Regulator MumR Protects Acinetobacter baumannii from Oxidative Stress.Infection and immunity · 2020Article
- The interplay between Mn and Fe in Deinococcus radiodurans triggers cellular protection during paraquat-induced oxidative stress.Scientific reports · 2019Article
- Manganese co-localizes with calcium and phosphorus inThe Journal of biological chemistry · 2019Article
- Cellular maturation of an iron-type nitrile hydratase interrogated using EPR spectroscopy.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2019Article
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Abstract
The remarkable ability of bacterium Deinococcus radiodurans to survive extreme doses of γ-rays (12,000 Gy), 20 times greater than Escherichia coli, is undiminished by loss of Mn-dependent superoxide dismutase (SodA). D. radiodurans radiation resistance is attributed to the accumulation of low-molecular-weight (LMW) "antioxidant" Mn(2+)-metabolite complexes that protect essential enzymes from oxidative damage. However, in vivo information about such complexes within D. radiodurans cells is lacking, and the idea that they can supplant reactive-oxygen-species (ROS)-scavenging enzymes remains controversial. In this report, measurements by advanced paramagnetic resonance techniques [electron-spin-echo (ESE)-EPR/electron nuclear double resonance/ESE envelope modulation (ESEEM)] reveal differential details of the in vivo Mn(2+) speciation in D. radiodurans and E. coli cells and their responses to 10 kGy γ-irradiation. The Mn(2+) of D. radiodurans exists predominantly as LMW complexes with nitrogenous metabolites and orthophosphate, with negligible EPR signal from Mn(2+) of SodA. Thus, the extreme radiation resistance of D. radiodurans cells cannot be attributed to SodA. Correspondingly, 10 kGy irradiation causes no change in D. radiodurans Mn(2+) speciation, despite the paucity of holo-SodA. In contrast, the EPR signal of E. coli is dominated by signals from low-symmetry enzyme sites such as that of SodA, with a minority pool of LMW Mn(2+) complexes that show negligible coordination by nitrogenous metabolites. Nonetheless, irradiation of E. coli majorly changes LMW Mn(2+) speciation, with extensive binding of nitrogenous ligands created by irradiation. We infer that E. coli is highly susceptible to radiation-induced ROS because it lacks an adequate supply of LMW Mn antioxidants.
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