ArticleDiabetologia2013
Oscillations of sub-membrane ATP in glucose-stimulated beta cells depend on negative feedback from Ca(2+).
Article in Diabetologia, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
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Who cites it
54 citing papers in PubMed, 92 citations in OpenAlex.
- GABA Regulates CaMetabolites · 2026Article
- The Dual Anaplerotic Model (DAM): Integral Roles of Pyruvate Carboxylase and the GABA Shunt in Beta Cell Insulin Secretion.Life (Basel, Switzerland) · 2026Article
- Mechanistic insights and approaches for beta cell regeneration.Nature chemical biology · 2025Review
- The Synergistic Impact of Glycolysis, Mitochondrial OxPhos, and PEP Cycling on ATP Production in Beta Cells.International journal of molecular sciences · 2025Article
- Article
- Do We Need a New Hypothesis for KATP Closure in β-Cells? Distinguishing the Baby From the Bathwater.Diabetes · 2024Article
- Review
- Review
- TALK-1-mediated alterations of β-cell mitochondrial function and insulin secretion impair glucose homeostasis on a diabetogenic diet.Cell reports · 2024Article
- A primer on modelling pancreatic islets: from models of coupled β-cells to multicellular islet models.Islets · 2023Review
- KThe Journal of physiology · 2023Article
- Deconstructing the integrated oscillator model for pancreatic β-cells.Mathematical biosciences · 2023Article
- CaAmerican journal of physiology. Endocrinology and metabolism · 2023Review
- Liraglutide increases islet CaDiabetes, obesity & metabolism · 2022Article
- Metabolic cycles and signals for insulin secretion.Cell metabolism · 2022Review
- Pulsatile Basal Insulin Secretion Is Driven by Glycolytic Oscillations.Physiology (Bethesda, Md.) · 2022Review
- Oscillations in K(ATP) conductance drive slow calcium oscillations in pancreatic β-cells.Biophysical journal · 2022Article
- Slow oscillations persist in pancreatic beta cells lacking phosphofructokinase M.Biophysical journal · 2022Article
- Mitochondrial clearance of calcium facilitated by MICU2 controls insulin secretion.Molecular metabolism · 2021Article
- The effects of beta-cell mass and function, intercellular coupling, and islet synchrony on [Formula: see text] dynamics.Scientific reports · 2021Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aims/hypothesisATP links changes in glucose metabolism to electrical activity, Ca(2+) signalling and insulin secretion in pancreatic beta cells. There is evidence that beta cell metabolism oscillates, but little is known about ATP dynamics at the plasma membrane, where regulation of ion channels and exocytosis occur.
methodsThe sub-plasma-membrane ATP concentration ([ATP]pm) was recorded in beta cells in intact mouse and human islets using total internal reflection microscopy and the fluorescent reporter Perceval.
resultsGlucose dose-dependently increased [ATP]pm with half-maximal and maximal effects at 5.2 and 9 mmol/l, respectively. Additional elevations of glucose to 11 to 20 mmol/l promoted pronounced [ATP]pm oscillations that were synchronised between neighbouring beta cells. [ATP]pm increased further and the oscillations disappeared when voltage-dependent Ca(2+) influx was prevented. In contrast, K(+)-depolarisation induced prompt lowering of [ATP]pm. Simultaneous recordings of [ATP]pm and the sub-plasma-membrane Ca(2+) concentration ([Ca(2+)]pm) during the early glucose-induced response revealed that the initial [ATP]pm elevation preceded, and was temporarily interrupted by the rise of [Ca(2+)]pm. During subsequent glucose-induced oscillations, the increases of [Ca(2+)]pm correlated with lowering of [ATP]pm. CONCLUSIONS/
interpretationIn beta cells, glucose promotes pronounced oscillations of [ATP]pm, which depend on negative feedback from Ca(2+) . The bidirectional interplay between these messengers in the sub-membrane space generates the metabolic and ionic oscillations that underlie pulsatile insulin secretion.
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