ArticleParasitology research2013
Efficient capture of pathogens with a zeolite matrix.
Article in Parasitology research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
4 citing papers in PubMed, 12 citations in OpenAlex.
- Biomedical applications of solid-binding peptides and proteins.Materials today. Bio · 2023Review
- Universal Enzyme-Based Field Workflow for Rapid and Sensitive Quantification of Water Pathogens.Microorganisms · 2021Article
- Solid-binding peptides for immobilisation of thermostable enzymes to hydrolyse biomass polysaccharides.Biotechnology for biofuels · 2017Article
- A Novel Universal Detection Agent for Time-Gated Luminescence Bioimaging.Scientific reports · 2016Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We show that a peptide linker sequence expressed as part of a fusion with protein G from Streptococcus binds to natural zeolite giving a complex to which a suitable antibody shows affinity binding. As a consequence, addition of the CRY104 antibody specific for an external surface epitope of Cryptosporidium allows the capture of this protozoan pathogen at high efficiency with the advantage of rapid concentration from water samples. The natural zeolite with the specific antibody attached was incorporated into a single and double zeolite column system. The results reported here show that it is possible to capture Cryptosporidium oocysts with outstanding efficiency (>90 %) from water and water incorporating QC-MUD using a size-sorted natural zeolite. The natural zeolite matrix not only allows for high flow rates but also for flexibility of column design and volume of water for sample collection. The system is versatile and it is possible to prepare columns with more than one specific surface antibody to allow the capture of one or two pathogens simultaneously.
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Registered trials
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