ArticleScientific reports2013
Establishment of a versatile cell line for juvenile hormone signaling analysis in Tribolium castaneum.
Article in Scientific reports, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 81 citations in OpenAlex.
- Juvenile hormone receptor relies on conserved domain interfaces for dimerization-dependent activity.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Establishment of 27 cell lines derived from various insects.In vitro cellular & developmental biology. Animal · 2026Article
- Evolution of environmental sex determination via juvenile hormone-induced gene co-option inProceedings of the National Academy of Sciences of the United States of America · 2026Article
- The Juvenile-Hormone-Responsive FactorBiomolecules · 2023Article
- A database of crop pest cell lines.In vitro cellular & developmental biology. Animal · 2022Article
- A new continuous cell line from the pest insect, Anomala cuprea (Coleoptera; Scarabaeidae): emergence of contractile cells.In vitro cellular & developmental biology. Animal · 2022Article
- Review
- Purification of an insect juvenile hormone receptor complex enables insights into its post-translational phosphorylation.The Journal of biological chemistry · 2021Article
- Detection of juvenile hormone agonists by a new reporter gene assay using yeast expressing Drosophila methoprene-tolerant.FEBS open bio · 2021Article
- Identification of juvenile hormone-induced posttranslational modifications of methoprene tolerant and Krüppel homolog 1 in the yellow fever mosquito, Aedes aegypti.Journal of proteomics · 2021Article
- Reporter gene assays for screening and identification of novel molting hormone- and juvenile hormone-like chemicals.Journal of pesticide science · 2021Article
- Identification of novel juvenile-hormone signaling activatorsJournal of pesticide science · 2021Article
- A simple method for ex vivo honey bee cell culture capable of in vitro gene expression analysis.PloS one · 2021Article
- Identification of a juvenile-hormone signaling inhibitor via high-throughput screening of a chemical library.Scientific reports · 2020Article
- Establishment and characterization of novel cell lines derived from six lepidopteran insects collected in the field.In vitro cellular & developmental biology. Animal · 2020Article
- Article
- Synergistic action of the transcription factors Krüppel homolog 1 and Hairy in juvenile hormone/Methoprene-tolerant-mediated gene-repression in the mosquito Aedes aegypti.PLoS genetics · 2019Article
- Exquisite ligand stereoselectivity of aThe Journal of biological chemistry · 2019Article
- Epigenetic modifications acetylation and deacetylation play important roles in juvenile hormone action.BMC genomics · 2018Article
- Insect Transcription Factors: A Landscape of Their Structures and Biological Functions inInternational journal of molecular sciences · 2018Review
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The red flour beetle, Tribolium castaneum, has been widely used as a laboratory model for analyzing gene function. In this study, we established a novel cell line (Tc81) from T. castaneum embryos and validated the utility of this cell line by analyzing the juvenile hormone (JH) signaling pathway. In Tc81 cells, the Krüppel homolog 1 gene (Kr-h1), which is a JH-dependent repressor of insect metamorphosis, was rapidly induced by subnanomolar levels of JHs. Bioinformatics analysis and reporter assays identified 2 JH response elements (kJHREs) located in the region upstream of the transcription start site and in the first intron of Kr-h1. Furthermore, methoprene tolerant (Met) and steroid receptor co-activator (SRC) RNAi reduced JH-dependent induction of Kr-h1 transcripts and kJHRE-reporter activities. Thus, this novel Tc81 cell line is useful for the elucidation of JH signaling and is a promising tool for the functional analysis of genes by RNAi and reporter assays.
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