ArticleScientific reports2013
Long-term strain improvements accumulate mutations in regulatory elements responsible for hyper-production of cellulolytic enzymes.
Article in Scientific reports, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed, 110 citations in OpenAlex.
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- Mechanisms of Enhanced Low-Temperature Lignocellulose Degradation by an ARTP-InducedMicroorganisms · 2026Article
- The Transcription Factor PoCon7 Is Essential for Fungal Viability and Regulates Chitinase Gene Expression inInternational journal of molecular sciences · 2025Article
- Review
- Modification of essential factors mediating post-translational processing for high-quality protein expression inEngineering microbiology · 2025Article
- Discovery of a novel translation-machinery-associated protein that positively correlates with cellulase production.Biotechnology for biofuels and bioproducts · 2025Article
- Heterochromatin Protein Activates the Amylase Expression Pathway and Its Application to Recombinant Protein Expression in Penicillium oxalicum.Current microbiology · 2025Article
- Regulation of genes encoding polysaccharide-degrading enzymes in Penicillium.Applied microbiology and biotechnology · 2024Review
- Characterization of yeast mutant strains for starter culture in Arabica coffee fermentation.Scientific reports · 2024Article
- Different Putative Methyltransferases Have Different Effects on the Expression Patterns of Cellulolytic Genes.Journal of fungi (Basel, Switzerland) · 2023Article
- Regulation of nutrient utilization in filamentous fungi.Applied microbiology and biotechnology · 2023Review
- Preparation of cellulose nanocrystals from commercial dissolving pulp using an engineered cellulase system.Bioresources and bioprocessing · 2023Article
- Dynamic Microstructure Assembly Driven byMicroorganisms · 2022Article
- Construction of a novel filamentous fungal protein expression system based on redesigning of regulatory elements.Applied microbiology and biotechnology · 2022Article
- Carbon catabolite repression involves physical interaction of the transcription factor CRE1/CreA and the Tup1-Cyc8 complex in Penicillium oxalicum and Trichoderma reesei.Biotechnology for biofuels · 2021Article
- CRISPR/Cas9-mediated genome editing in Penicillium oxalicum and Trichoderma reesei using 5S rRNA promoter-driven guide RNAs.Biotechnology letters · 2021Article
- Constructing a novel expression system by specific activation of amylase expression pathway in Penicillium.Microbial cell factories · 2020Article
- The Role of Cross-Pathway Control Regulator CpcA in the Growth and Extracellular Enzyme Production of Penicillium oxalicum.Current microbiology · 2020Article
- Involvement ofFrontiers in microbiology · 2020Article
Corrections and comments
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Authors and funding
28 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Long-term strain improvements through repeated mutagenesis and screening have generated a hyper-producer of cellulases and hemicellulases from Penicillium decumbens 114 which was isolated 30 years ago. Here, the genome of the hyper-producer P. decumbens JU-A10-T was sequenced and compared with that of the wild-type strain 114-2. Further, the transcriptomes and secretomes were compared between the strains. Selective hyper-production of cellulases and hemicellulases but not all the secreted proteins was observed in the mutant, making it a more specific producer of lignocellulolytic enzymes. Functional analysis identified that changes in several transcriptional regulatory elements played crucial roles in the cellulase hyper-producing characteristics of the mutant. Additionally, the mutant showed enhanced supply of amino acids and decreased synthesis of secondary metabolites compared with the wild-type. The results clearly point out that we can target gene regulators and promoters with minimal alterations of the genetic content but maximal effects in genetic engineering.
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