ArticleMolecular pharmaceutics2013
Polyarginine molecular weight determines transfection efficiency of calcium condensed complexes.
Article in Molecular pharmaceutics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Polypeptide-Based Systems: From Synthesis to Application in Drug Delivery.Pharmaceutics · 2023Review
- Nona-Arginine Mediated Anti-E6 ShRNA Delivery Suppresses the Growth of Hela Cells in vitro.Iranian biomedical journal · 2023Article
- Structural control of self-assembled peptide nanostructures to develop peptide vesicles for photodynamic therapy of cancer.Materials today. Bio · 2022Article
- Benefit of a Short Chain Peptide as a Targeting Ligand of Nanocarriers for a Brain-Driven Purpose.Pharmaceutics · 2021Article
- RNAi inACS applied materials & interfaces · 2020Article
- Lipofection with estrone-based luminophores featuring aggregation-induced emission properties.RSC advances · 2020Article
- Polyamide Backbone Modified Cell Targeting and Penetrating Peptides in Cancer Detection and Treatment.Frontiers in chemistry · 2020Review
- Cell Penetrating Peptides as Molecular Carriers for Anti-Cancer Agents.Molecules (Basel, Switzerland) · 2018Review
- Antibody-mediated oral delivery of therapeutic DNA for type 2 diabetes mellitus.Biomaterials research · 2018Article
- Combined Local Pulmonary and Systemic Delivery of AT2R Gene by Modified TAT Peptide Nanoparticles Attenuates Both Murine and Human Lung Carcinoma Xenografts in Mice.Journal of pharmaceutical sciences · 2017Article
- Targeted gene silencing of CCL2 inhibits triple negative breast cancer progression by blocking cancer stem cell renewal and M2 macrophage recruitment.Oncotarget · 2016Article
- AT2R Gene Delivered by Condensed Polylysine Complexes Attenuates Lewis Lung Carcinoma after Intravenous Injection or Intratracheal Spray.Molecular cancer therapeutics · 2016Article
- Electrostatic Localization of RNA to Protocell Membranes by Cationic Hydrophobic Peptides.Angewandte Chemie (International ed. in English) · 2015Article
- Effect of lipid headgroup charge and pH on the stability and membrane insertion potential of calcium condensed gene complexes.Langmuir : the ACS journal of surfaces and colloids · 2015Article
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2 authors.
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Abstract
Cell penetrating peptides (CPPs) have been extensively studied in polyelectrolyte complexes as a means to enhance the transfection efficiency of plasmid DNA (pDNA). Increasing the molecular weight of CPPs often enhances gene expression but poses a risk of increased cytotoxicity and immunogenicity compared to low molecular weight CCPs. Conversely, low molecular weight CPPs typically have low transfection efficiency due to large complex size. Complexes made using low molecular weight CPPs were found to be condensed to a small size by adding calcium. In this study, complexes of low molecular weight polyarginine and pDNA were condensed with calcium. These complexes showed high transfection efficiency and low cytotoxicity in A549 carcinomic human alveolar basal epithelial cells. The relationships between transfection efficiency and polyarginine size (5, 7, 9, or 11 amino acids), polyarginine/pDNA charge ratios, and calcium concentrations were studied. Polyarginine 7 was significantly more effective than other polyarginines under most formulation conditions, suggesting a link between cell penetration ability and transfection efficiency.
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