Trial reportThe Journal of biological chemistry2013
β2 integrin-mediated cell-cell contact transfers active myeloperoxidase from neutrophils to endothelial cells.
Trial report in The Journal of biological chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
33 citing papers in PubMed, 58 citations in OpenAlex.
- Trial
- Transcriptional meta-analysis and immune cell profiles reveal altered neutrophil dynamics in chronic atrial fibrillation.Royal Society open science · 2025Article
- Elevated circulating levels of neutrophil extracellular traps after cardiopulmonary bypass surgery as risk factors of postoperative atrial fibrillation and mortality.Journal of thoracic disease · 2024Article
- Neutrophils insert elastase into hepatocytes to regulate calcium signaling in alcohol-associated hepatitis.The Journal of clinical investigation · 2024Article
- Extracellular Vesicle ITGAM and ITGB2 Mediate Severe Acute Pancreatitis-Related Acute Lung Injury.ACS nano · 2023Article
- Characterization of Myeloperoxidase in the Healthy Equine Endometrium.Animals : an open access journal from MDPI · 2023Article
- Article
- Myeloperoxidase: Regulation of Neutrophil Function and Target for Therapy.Antioxidants (Basel, Switzerland) · 2022Review
- Myeloperoxidase immunohistochemical staining can identify glomerular endothelial cell injury in dense deposit disease.Pediatric nephrology (Berlin, Germany) · 2021Article
- Extracellular vesicles mediate cellular interactions in renal diseases-Novel views of intercellular communications in the kidney.Journal of cellular physiology · 2021Review
- β2 Integrin Regulation of Neutrophil Functional Plasticity and Fate in the Resolution of Inflammation.Frontiers in immunology · 2021Review
- Extracellular vesicles and lupus nephritis - New insights into pathophysiology and clinical implications.Journal of autoimmunity · 2020Review
- Endothelial dysfunction in neuroprogressive disorders-causes and suggested treatments.BMC medicine · 2020Review
- The complexity of neutrophils in health and disease: Focus on cancer.Seminars in immunology · 2020Review
- Bacteria evoke alarm behaviour in zebrafish.Nature communications · 2019Article
- Annexin A1-mediated inhibition of inflammatory cytokines may facilitate the resolution of inflammation in acute radiation-induced lung injury.Oncology letters · 2019Article
- The role of monocytosis and neutrophilia in atherosclerosis.Journal of cellular and molecular medicine · 2018Review
- Neutrophils and PMN-MDSC: Their biological role and interaction with stromal cells.Seminars in immunology · 2018Review
- Myeloperoxidase: A new player in autoimmunity.Cellular immunology · 2017Review
- Characterization of the CD177 interaction with the ANCA antigen proteinase 3.Scientific reports · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
Atherosclerosis and vasculitis both feature inflammation mediated by neutrophil-endothelial cell (EC) contact. Neutrophil myeloperoxidase (MPO) can disrupt normal EC function, although the mechanism(s) by which MPO is transferred to ECs are unknown. We tested the hypothesis that close, β2 integrin-dependent neutrophil-EC contact mediates MPO transfer from neutrophils to ECs. We used sensitive MPO assays and flow cytometry to detect MPO in ECs and demonstrate that ECs acquired MPO when contacted by neutrophils directly but not when ECs and neutrophils were separated in Transwells. The transfer was dependent on neutrophil number, exposure time, and incubation temperature. Transfer occurred in several EC types, increased with endotoxin, was not accompanied by MPO release into the medium, and was not abrogated by inhibiting degranulation to secretagogues. Confocal microscopy showed MPO internalization by ECs with cytoplasmic and nuclear staining. Neutrophils and ECs formed intimate contact sites demonstrated by electron microscopy. Blocking CD11b or CD18 β2 integrin chains, or using neutrophils from CD11b gene-deleted mice, reduced MPO transfer. EC-acquired MPO was enzymatically active, as demonstrated by its ability to oxidize the fluorescent probe aminophenyl fluorescein in the presence of a hydrogen peroxide source. The data suggest an alternative to EC uptake of soluble MPO, namely the cell contact-dependent, β2 integrin-mediated transfer from neutrophils. The findings could be of therapeutic relevance in atherosclerosis and vasculitis.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.