ArticleThe Journal of biological chemistry2013
A novel automethylation reaction in the Aspergillus nidulans LaeA protein generates S-methylmethionine.
Article in The Journal of biological chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
36 citing papers in PubMed, 71 citations in OpenAlex.
- The discovery of pesticide lead compounds from microbial secondary metabolites.Journal of pesticide science · 2026Article
- Article
- The global regulator LaeA controls expression of genes involved in diverse primary and secondary metabolites biosynthesis and their secretion in fungi.Frontiers in microbiology · 2026Review
- Polyamine Induction of Secondary Metabolite Biosynthetic Genes in Fungi Is Mediated by Global Regulator LaeA and α-NAC Transcriptional Coactivator: Connection to Epigenetic Modification of Histones.Molecules (Basel, Switzerland) · 2025Review
- Histone H2B lysine 122 and lysine 130, as the putative targets of Penicillium oxalicum LaeA, play important roles in asexual development, expression of secondary metabolite gene clusters, and extracellular glycoside hydrolase synthesis.World journal of microbiology & biotechnology · 2024Article
- Different Putative Methyltransferases Have Different Effects on the Expression Patterns of Cellulolytic Genes.Journal of fungi (Basel, Switzerland) · 2023Article
- PrJournal of fungi (Basel, Switzerland) · 2023Article
- Light regulation of secondary metabolism in fungi.Journal of biological engineering · 2023Review
- The putative methyltransferase LaeA regulates mycelium growth and cellulase production in Myceliophthora thermophila.Biotechnology for biofuels and bioproducts · 2023Article
- The Role of Chromatin and Transcriptional Control in the Formation of Sexual Fruiting Bodies in Fungi.Microbiology and molecular biology reviews : MMBR · 2022Review
- Epipolythiodioxopiperazine-Based Natural Products: Building Blocks, Biosynthesis and Biological Activities.Chembiochem : a European journal of chemical biology · 2022Review
- LaeA regulates morphological development and ochratoxin A biosynthesis in Aspergillus niger.Mycotoxin research · 2022Article
- Arsenic 3 methyltransferase (AS3MT) automethylates on cysteine residues in vitro.Archives of toxicology · 2022Article
- The BcLAE1 is involved in the regulation of ABA biosynthesis inFrontiers in microbiology · 2022Article
- First report on the metabolic characterization of Sterigmatocystin production by selectFrontiers in microbiology · 2022Article
- Profiling of chromatin accessibility identifies transcription factor binding sites across the genome of Aspergillus species.BMC biology · 2021Article
- Heteroexpression ofMarine drugs · 2020Article
- Characterization of SETD3 methyltransferase-mediated protein methionine methylation.The Journal of biological chemistry · 2020Article
- Article
- LaeA Controls Citric Acid Production through Regulation of the Citrate Exporter-EncodingApplied and environmental microbiology · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
The filamentous fungi in the genus Aspergillus are opportunistic plant and animal pathogens that can adapt to their environment by producing various secondary metabolites, including lovastatin, penicillin, and aflatoxin. The synthesis of these small molecules is dependent on gene clusters that are globally regulated by the LaeA protein. Null mutants of LaeA in all pathogenic fungi examined to date show decreased virulence coupled with reduced secondary metabolism. Although the amino acid sequence of LaeA contains the motifs characteristic of seven-β-strand methyltransferases, a methyl-accepting substrate of LaeA has not been identified. In this work we did not find a methyl-accepting substrate in Aspergillus nidulans with various assays, including in vivo S-adenosyl-[methyl-(3)H]methionine labeling, targeted in vitro methylation experiments using putative protein substrates, or in vitro methylation assays using whole cell extracts grown under different conditions. However, in each experiment LaeA was shown to self-methylate. Amino acid hydrolysis of radioactively labeled LaeA followed by cation exchange and reverse phase chromatography identified methionine as the modified residue. Point mutations show that the major site of modification of LaeA is on methionine 207. However, in vivo complementation showed that methionine 207 is not required for the biological function of LaeA. LaeA is the first protein to exhibit automethylation at a methionine residue. These findings not only indicate LaeA may perform novel chemistry with S-adenosylmethionine but also provide new insights into the physiological function of LaeA.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.