ArticleInvestigative ophthalmology & visual science2013
Transcription factors Runx1 to 3 are expressed in the lacrimal gland epithelium and are involved in regulation of gland morphogenesis and regeneration.
Article in Investigative ophthalmology & visual science, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 41 citations in OpenAlex.
- Human Lacrimal Gland Gene Expression.PloS one · 2017Trial
- Advanced Biomaterials for Lacrimal Tissue Engineering: A Review.Materials (Basel, Switzerland) · 2024Review
- RUN(X) out of blood: emerging RUNX1 functions beyond hematopoiesis and links to Down syndrome.Human genomics · 2023Review
- Article
- Distinct Inflammatory and Oxidative Effects of Diabetes Mellitus and Hypothyroidism in the Lacrimal Functional Unit.International journal of molecular sciences · 2023Article
- Lacrimal Gland Epithelial Cells Shape Immune Responses through the Modulation of Inflammasomes and Lipid Metabolism.International journal of molecular sciences · 2023Article
- Article
- Article
- Origin and Lineage Plasticity of Endogenous Lacrimal Gland Epithelial Stem/Progenitor Cells.iScience · 2020Article
- Establishment of long-term serum-free culture for lacrimal gland stem cells aiming at lacrimal gland repair.Stem cell research & therapy · 2020Article
- Lacrimal gland development: From signaling interactions to regenerative medicine.Developmental dynamics : an official publication of the American Association of Anatomists · 2017Review
- Manipulation of Panx1 Activity Increases the Engraftment of Transplanted Lacrimal Gland Epithelial Progenitor Cells.Investigative ophthalmology & visual science · 2017Article
- Strategies for Regenerating the Lacrimal Gland.Current ophthalmology reports · 2017Article
- Defining epithelial cell dynamics and lineage relationships in the developing lacrimal gland.Development (Cambridge, England) · 2017Article
- Runx1 mediates the development of the granular convoluted tubules in the submandibular glands.PloS one · 2017Article
- Lacrimal Gland Repair Using Progenitor Cells.Stem cells translational medicine · 2017Article
- Article
- Alteration in cellular turnover and progenitor cell population in lacrimal glands from thrombospondin 1Experimental eye research · 2016Article
- A microRNA/Runx1/Runx2 network regulates prostate tumor progression from onset to adenocarcinoma in TRAMP mice.Oncotarget · 2016Article
- Histatin-1 Expression in Human Lacrimal Epithelium.PloS one · 2016Article
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Authors and funding
7 authors at 4 institutions in 3 countries.
Funding
Abstract
purposeLacrimal gland (LG) morphogenesis and repair are regulated by a complex interplay of intrinsic factors (e.g., transcription factors) and extrinsic signals (e.g., soluble growth/signaling factors). Many of these interconnections remain poorly characterized. Runt-related (Runx) factors belong to a small family of heterodimeric transcription factors known to regulate lineage-specific proliferation and differentiation of stem cells. The purpose of this study was to define the expression pattern and the role of Runx proteins in LG development and regeneration.
methodsExpression of epithelial-restricted transcription factors in murine LG was examined using immunostaining, qRT-PCR, and RT(2)Profiler PCR microarrays. The role of Runx transcription factors in LG morphogenesis was studied using siRNA and ex vivo LG cultures. Expression of Runx transcription factors during LG regeneration was assessed using in vivo model of LG regeneration.
resultsWe found that Runx factors are expressed in the epithelial compartment of the LG; in particular, Runx1 was restricted to the epithelium with highest level of expression in ductal and centroacinar cells. Downregulation of Runx1 to 3 expression using Runx-specific siRNAs abolished LG growth and branching and our data suggest that Runx1, 2, and 3 are partially redundant in LG development. In siRNA-treated LG, reduction of branching correlated with reduction of epithelial proliferation, as well as expression of cyclin D1 and the putative epithelial progenitor cell marker cytokeratin-5. Runx1, Runx3, and cytokeratin-5 expression increased significantly in regenerating LG and there was modest increase in Runx2 expression during LG differentiation.
conclusionsRunx1 and 2 are new markers of the LG epithelial lineage and Runx factors are important for normal LG morphogenesis and regeneration.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.