ArticleAnnals of botany2013
DNA profiling, telomere analysis and antioxidant properties as tools for monitoring ex situ seed longevity.
Article in Annals of botany, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 66 citations in OpenAlex.
- Seed Longevity and Ageing: A Review on Physiological and Genetic Factors with an Emphasis on Hormonal Regulation.Plants (Basel, Switzerland) · 2023Review
- Genomic Identification and Expression Profiling of Lesion Simulating Disease Genes in Alfalfa (Antioxidants (Basel, Switzerland) · 2023Article
- Comparative Seeds Storage Transcriptome Analysis ofInternational journal of molecular sciences · 2022Article
- A Critical Review of the Current Global Ex Situ Conservation System for Plant Agrobiodiversity. II. Strengths and Weaknesses of the Current System and Recommendations for Its Improvement.Plants (Basel, Switzerland) · 2021Review
- Why Seed Physiology Is Important for Genebanking.Plants (Basel, Switzerland) · 2020Review
- Genetic and Epigenetic Stability in Rye Seeds under Different Storage Conditions: Ageing and Oxygen Effect.Plants (Basel, Switzerland) · 2020Article
- Hydropriming and Biopriming ImproveGenes · 2020Article
- Aspergillus fumigatus and Aspergillosis in 2019.Clinical microbiology reviews · 2019Review
- Hydrogen peroxide signaling integrates with phytohormones during the germination of magnetoprimed tomato seeds.Scientific reports · 2019Article
- Systems biology and genome-wide approaches to unveil the molecular players involved in the pre-germinative metabolism: implications on seed technology traits.Plant cell reports · 2017Review
- The Seed Repair Response during Germination: Disclosing Correlations between DNA Repair, Antioxidant Response, and Chromatin Remodeling inFrontiers in plant science · 2017Article
- Seed birth to death: dual functions of reactive oxygen species in seed physiology.Annals of botany · 2015Review
- Seed priming: state of the art and new perspectives.Plant cell reports · 2015Review
- Towards a better monitoring of seed ageing under ex situ seed conservation.Conservation physiology · 2015Article
- Analysis of the transgenerational iron deficiency stress memory in Arabidopsis thaliana plants.Frontiers in plant science · 2015Article
- Environmentally induced transgenerational changes in seed longevity: maternal and genetic influence.Annals of botany · 2014Article
- Advances in seed conservation of wild plant species: a review of recent research.Conservation physiology · 2013Article
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
BACKGROUND AND
aimsThe germination test currently represents the most used method to assess seed viability in germplasm banks, despite the difficulties caused by the occurrence of seed dormancy. Furthermore, seed longevity can vary considerably across species and populations from different environments, and studies related to the eco-physiological processes underlying such variations are still limited in their depth. The aim of the present work was the identification of reliable molecular markers that might help in monitoring seed deterioration.
methodsDry seeds were subjected to artificial ageing and collected at different time points for molecular/biochemical analyses. DNA damage was measured using the RAPD (random amplified polymorphic DNA) approach while the seed antioxidant profile was obtained using both the DPPH (1,1-diphenyl, 2-picrylhydrazyl) assay and the Folin-Ciocalteu reagent method. Electron paramagnetic resonance (EPR) provided profiles of free radicals. Quantitative real-time polymerase chain reaction (QRT-PCR) was used to assess the expression profiles of the antioxidant genes MT2 (type 2 metallothionein) and SOD (superoxide dismutase). A modified QRT-PCR protocol was used to determine telomere length. KEY
resultsThe RAPD profiles highlighted different capacities of the two Silene species to overcome DNA damage induced by artificial ageing. The antioxidant profiles of dry and rehydrated seeds revealed that the high-altitude taxon Silene acaulis was characterized by a lower antioxidant specific activity. Significant upregulation of the MT2 and SOD genes was observed only in the rehydrated seeds of the low-altitude species. Rehydration resulted in telomere lengthening in both Silene species.
conclusionsDifferent seed viability markers have been selected for plant species showing inherent variation of seed longevity. RAPD analysis, quantification of redox activity of non-enzymatic antioxidant compounds and gene expression profiling provide deeper insights to study seed viability during storage. Telomere lengthening is a promising tool to discriminate between short- and long-lived species.
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