ArticleFluids and barriers of the CNS2013
The hCMEC/D3 cell line as a model of the human blood brain barrier.
Article in Fluids and barriers of the CNS, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 376 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
376 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Meta-analysis of RNA-seq Data Identifies Involvement of Interferon-Induced Genes to Facilitate Blood-Brain Barrier Traversal of Neuroinvasive Pathogens.Journal of molecular neuroscience : MN · 2025Pooled it
- Adipose tissue-derived small extracellular vesicles and blood-brain barrier function in adults with overweight and obesity.Experimental physiology · 2026Trial
- Article
- Cetuximab-loaded self-assembled polymeric nanoparticles for the integrin-targeted therapy of glioblastoma.JCIS open · 2026Article
- Biomedical Materials and Fabrication Methods for Construction of In Vitro Neurovascular Unit Models.Materials (Basel, Switzerland) · 2026Review
- vascr: An R-based toolkit for rapid and robust analysis of cellular impedance sensing data.European biophysics journal : EBJ · 2026Article
- Article
- Blood‒brain barrier permeability assessment: from biomimetic models to multimodal imaging.Journal of Zhejiang University. Science. B · 2026Review
- Refined single-cell profiling captures a CCR5EBioMedicine · 2026Article
- Establishment and Optimization of a Human Flow-Based Hollow FiberPharmaceutics · 2026Article
- Modeling blood-brain barrier-glioblastoma interactions: implications for chemoresistance and therapeutic targeting.Fluids and barriers of the CNS · 2026Review
- A Physiological Microfluidic Blood-Brain-Barrier Model for In Vitro Study of Nanoparticle Trafficking and Accumulation.Advanced healthcare materials · 2026Article
- Article
- Accelerated reprogramming of hiPSCs into functional brain endothelial-like cells using multiplexed CRISPR activation.Scientific reports · 2026Article
- CRISPR interference in aJournal of bacteriology · 2026Article
- Shiga Toxin Induces ApoptosisJournal of microbiology and biotechnology · 2026Article
- Unveiling the Impact ofJournal of the American Heart Association · 2026Article
- Polymeric-Based Theranostic Nanocarriers of Neuroprotective Drugs: Development, Imaging, and Bioanalysis.Molecular pharmaceutics · 2026Article
- Influence of surface characteristics on the in vitro stability and cell uptake of nanoliposomes for brain delivery.Beilstein journal of nanotechnology · 2026Article
- Article
316 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Since the first attempts in the 1970s to isolate cerebral microvessel endothelial cells (CECs) in order to model the blood-brain barrier (BBB) in vitro, the need for a human BBB model that closely mimics the in vivo phenotype and is reproducible and easy to grow, has been widely recognized by cerebrovascular researchers in both academia and industry. While primary human CECs would ideally be the model of choice, the paucity of available fresh human cerebral tissue makes wide-scale studies impractical. The brain microvascular endothelial cell line hCMEC/D3 represents one such model of the human BBB that can be easily grown and is amenable to cellular and molecular studies on pathological and drug transport mechanisms with relevance to the central nervous system (CNS). Indeed, since the development of this cell line in 2005 over 100 studies on different aspects of cerebral endothelial biology and pharmacology have been published. Here we review the suitability of this cell line as a human BBB model for pathogenic and drug transport studies and we critically consider its advantages and limitations.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.